0000000000241852
AUTHOR
A. Sicilia
Collecting and preserving plant DNA for huanglongbing diagnosis in citrus samples from China
Accurate and sensitive detection of Citrus Huanglongbing associated ‘Ca. Liberibacter’species, not currently reported in the European and Mediterranean area, is an imperative need to define certification programs, to prevent introduction of the bacteria and/or their vectors in the unaffected areas, and to delineate efficient management strategies in those areas where the disease has spread. In this study, we compared different citrus sample preparation procedures for PCR based detection methods of ‘Candidatus Liberibacter asiaticus’, in order to find out the best a way to transport and preserve samples of Shatangju mandarin and fingered citron obtained during a survey in citrus orchards in …
Contribution to the knowledge of Tunisian amphibians: notes on distribution, habitat features and breeding phenology
In order to improve the knowledge on amphibian fauna in Tunisia, about 300 water bodies belonging to different habitat typologies were sampled during the period 2004-2008. At the sites where amphibians were observed, we recorded the UTM coordinates, the altitude, and the following environmental features: habitat typology, hydroperiod, electric conductivity, temperature, water turbidity, and macrophyte coverage. The presence of the seven species currently known with certainty for Tunisia has been confirmed: Pleurodeles nebulosus, Discoglossus pictus, Bufo bufo, B. mauritanicus, B. boulengeri, Hyla meridionalis and Pelophylax saharicus. Conversely, no evidence on the occurrence of Salamandra …