0000000000242761

AUTHOR

Francesca Scarnicci

showing 4 related works from this author

The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

2014

The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. …

DNA depurination; Forensic genetics; PCR fidelity; STR typing; Biochemistry; Clinical BiochemistryPCR fidelityGenotyping TechniquesDNA damageSample (material)Clinical BiochemistryDNA depurinationReproducibility of ResultForensic geneticsBiologyPolymerase Chain ReactionBiochemistryNOAnalytical Chemistrylaw.inventionDNA depurination; PCR fidelity; STR typing; forensic genetics.Settore MED/43 - Medicina LegalelawSettore BIO/13 - Biologia ApplicataGenotypeHumansSTR typingGenotyping TechniquesPolymerase chain reactionProtocol (science)GeneticsMedicine (all)Reproducibility of ResultsForensic geneticDNAAmpliconDNA FingerprintingDNA depurination; Forensic genetics; PCR fidelity; STR typingSettore BIO/18 - GeneticaDNA depurination Forensic genetics PCR fidelity STR typingDNA profilingSettore MED/03 - Genetica MedicaMicrosatellite RepeatGenotyping TechniqueDNA depurination; Forensic genetics; PCR fidelity; STR typing;Microsatellite RepeatsHuman
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Moors and Saracens in Europe: estimating the medieval North African male legacy in southern Europe.

2009

To investigate the male genetic legacy of the Arab rule in southern Europe during medieval times, we focused on specific Northwest African haplogroups and identified evolutionary close STR-defined haplotypes in Iberia, Sicily and the Italian peninsula. Our results point to a higher recent Northwest African contribution in Iberia and Sicily in agreement with historical data, southern Italian regions known to have experienced long-term Arab presence also show an enrichment of Northwest African types. The forensic and genomic implications of these findings are discussed.

MaleY chromosome north africa medieval legacyPopulationShort ReportNorth africaHaplogroupZoological sciencesEvolution MolecularMoorsAfrica NorthernPeninsulaSettore BIO/13 - Biologia ApplicataHumansgeneticseducationGenetics (clinical)education.field_of_studygeography.geographical_feature_categoryChromosomes Human YGeographyEvolution (zoology)social sciencesSettore MED/43 - MEDICINA LEGALEpopulationseye diseasesArabsEuropeGeographyGenetics PopulationHaplotypesAnthropologySaracenEthnologyNorth africangeographic locationsEuropean journal of human genetics : EJHG
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WITHDRAWN: Corrigendum to ‘Development of an Italian RM Y-STR haplotype database: results of the 2013 GEFI collaborative exercise’ [Forensic. Sci. In…

2018

An inconsistency in the nomenclature used for the rapidly mutating (RM) Y-chromosomal short tandem repeat (Y-STR) marker DYS449 was noted in the above paper. In this paper, the DYS449 allele nomenclature introduced by Ballantyne et al. was used, instead of that described by Redd et al. and subsequently adopted by the International RM Y-STR User Group and in the AMPFlSTR® YFiler Plus kit.

GeneticsUser groupHaplotypeGeneticsY-STRBiologyPathology and Forensic MedicineForensic Science International: Genetics
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Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise.

2015

Recently introduced rapidly mutating Y-chromosomal short tandem repeat (RM Y-STR) loci, displaying a multiple-fold higher mutation rate relative to any other Y-STRs, including those conventionally used in forensic casework, have been demonstrated to improve the resolution of male lineage differentiation and to allow male relative separation usually impossible with standard Y-STRs. However, large and geographically-detailed frequency haplotype databases are required to estimate the statistical weight of RM Y-STR haplotype matches if observed in forensic casework. With this in mind, the Italian Working Group (GEFI) of the International Society for Forensic Genetics launched a collaborative ex…

Quality ControlMutation rateRegional ItalianLineage differentiationDNA PrimerY-chromosome; Rapidly mutating Y-STRs (RM Y-STRs); Haplotype; Lineage differentiation; Relative differentiation; Italy2734Biologycomputer.software_genrePathology and Forensic MedicineGeneticDatabases GeneticGeneticsHaplotype Italy Lineage differentiation Rapidly mutating Y-STRs (RM Y-STRs) Relative differentiation Y-chromosomeHaplotypeHumansY-STRCooperative BehaviorY-chromosomeDNA PrimersChromosomes Human YDatabaseBase SequenceMedicine (all)HaplotypeRelative differentiationhumanitiesForensic scienceHaplotypesItalyLineage differentiationMicrosatelliteRapidly mutating Y-STRs (RM Y-STRs)Haplotype estimationcomputerHumanForensic science international. Genetics
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