0000000000243863

AUTHOR

Gabriele Hill

showing 2 related works from this author

Nitric oxide is formed in a subpopulation of rat pineal cells and acts as an intercellular messenger.

1998

In the rat pineal, formation of the second messenger cyclic GMP (cGMP) is under adrenergic control. Two important sequential steps mediate adrenergic signal transduction by cGMP, receptor-stimulated nitric oxide (NO) formation by the enzyme NO synthase I (NOS I), and NO-induced cGMP formation by the cytosolic enzyme guanylyl cyclase. With regard to the first step in cGMP transduction (i.e. NO formation) we found, by means of NOS I immunostaining and NADPH-diaphorase staining, that the presence of NOS I was restricted to a subpopulation of pineal cells, generally surrounded by NOS I-negative cells. Considering the fact that NO is able to permeate the cell membrane, the question arises whethe…

Malemedicine.medical_specialtyAdrenergic receptorEndocrinology Diabetes and MetabolismAdrenergicBiologyNitric OxidePineal GlandSecond Messenger SystemsNitric oxideCell membraneRats Sprague-DawleyCellular and Molecular Neurosciencechemistry.chemical_compoundPineal glandEndocrinologyInternal medicineReceptors Adrenergic betamedicineAnimalsCyclic GMPEndocrine and Autonomic SystemsNADPH DehydrogenaseReceptors Adrenergic alphaImmunohistochemistryRatsCytosolmedicine.anatomical_structureEndocrinologychemistryGuanylate CyclaseOxyhemoglobinsSecond messenger systemSignal transductionNitric Oxide SynthaseSignal TransductionNeuroendocrinology
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In rat pinealocytes the cyclic GMP response to NO is regulated by Ca2+ and protein kinase C

1995

There is ample evidence that beta-adrenergic stimulation of cyclic GMP formation is potentiated by alpha1-adrenergic mechanisms, the latter leading to elevation of intracellular Ca2+ concentration ([Ca2+]i) and protein kinase C (PKC) activation. Recent studies have shown that nitric oxide synthase (NOS) and nitric oxide (NO) are a component of the adrenoceptor-cyclic GMP signalling pathway. The aim of the present investigation was to study the roles of alpha1-adrenergic mechanisms, Ca2+ and PKC on NO-stimulated cyclic GMP formation. To this end suspension cultures of rat pinealocytes were treated with the NO donor sodium nitroprusside (SNP) in the presence of alpha1-adrenergic agonists, [Ca…

MaleNitroprussidePhosphodiesterase InhibitorsNitric OxidePineal GlandPinealocyteNitric oxideRats Sprague-DawleyPhenylephrinechemistry.chemical_compoundCalmodulinmedicineAnimalsOuabainCyclic GMPProtein Kinase InhibitorsMolecular BiologyProtein Kinase CProtein kinase CbiologyKinaseGeneral NeuroscienceIsoproterenolPhosphodiesterasePhosphoric Monoester HydrolasesRatsNitric oxide synthaseCytosolBiochemistrychemistrybiology.proteinCalciumNeurology (clinical)Sodium nitroprussideDevelopmental Biologymedicine.drugBrain Research
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