Protein profiling analyses in arbuscular mycorrhizal symbiosis

Are diacylglycerol-kinases involved in cryptogein-induced early signaling events?

SPE IPM; National audience

Cellular and subcellular studies of the arbuscular mycorrhizal symbiosis in M. truncatula: a proteomic survey

Effets protecteurs de la symbiose mycorhizienne à arbuscule vis - à - vis du cadmium: identification des protéines potentiellement impliquées et quantification de deux transcrits

Studying the arbuscular mycorrhizal symbiosis from axenic system to soilborne interactions by proteomics: which challenges to face?

Blue-copper binding proteins of Medicago truncatula: Characterization of the expression of two genes related to the arbuscular mycorrhizal symbiosis

International audience; Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcpla and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the mycorrhizal development with Glomus intraradices for up to 7 weeks. Timecourse analysis in J5 showed that expression of both MtBcp1 genes increased continuously and correlated strongly with the colonization intensity and arbuscule content. MtPT4, selected as a reference gene of the functional plant-fungus association, showed a weaker correlation to mycorrhizal development. In a second experiment, a range of mycorr…

Mutations in DMI3 and SUNN modify the appressorium-responsive root proteome in arbuscular mycorrhiza.

Modification of the Medicago truncatula root proteome during the early stage of arbuscular mycorrhizal symbiosis was investigated by comparing, using two-dimensional electrophoresis, the protein patterns obtained from non-inoculated roots and roots synchronized for Glomus intraradices appressorium formation. This approach was conducted in wild-type (J5), mycorrhiza-defective (TRV25, dmi3), and autoregulation-defective (TR122, sunn) M. truncatula genotypes. The groups of proteins that responded to appressorium formation were further compared between wild-type and mutant genotypes; few overlaps and major differences were recorded, demonstrating that mutations in DMI3 and SUNN modified the ap…

Effect of cadmium on growth, isoflavonoid and protein accumulation patterns in mycorrhizal roots of Medicago truncatula

International audience; Ecosystems are submitted to various abiotic stresses, among which heavy metals represent major industrial pollutants. Cadmium (Cd), that has damaging effects on plant metabolism, occurs in agricultural environments through industrial pollution and human activities, including phosphate fertiliser and sewage sludge applications. Metal availability to plants can be modulated by soil microorganisms, including arbuscular mycorrhizal (AM) fungi. In the present work, Cd effects on the model legume Medicago truncatula inoculated or not with the AM fungus Glomus intraradices have been studied at 3 levels: (1) plant biomass production together with green part chlorophyll quant…

Germination and storage protein mobilisation of Trifolium subterraneum Var 45C seeds under hypoxic treatment

International audience; The rate, speed and latency period of germination were defined for seeds of Trifolium subterraneum variety 45 C. The latency period was 12 hours on distilled water. The hypoxic stress was applied at the beginning (T12) and the end of latency period (T24). It consists on the addition of nitrogen (N2) under 1 bar pressure in hermetically closed boxes during 2 hours. The germination of treated seeds was then assessed on distilled water. The germination test during hypoxic treatment showed that the hypoxic stress delayed germination, slowing down its speed and decreasing the final percentage of germinated seeds. In order to better understand this depressive effect observ…

Internalisation de la cryptogéine dans des cellules de tabac Nicotiana tabacum, cv Bright Yellow 2 (BY-2)

SPEBAPIPMUBINRA; Internalisation de la cryptogéine dans des cellules de tabac [i]Nicotiana tabacum[/i], cv Bright Yellow 2 (BY-2). 7. Journées Scientifiques et Techniques du Réseau des Microscopistes de l'INRA (RμI)

La tolérance au cadmium conférée par la symbiose endomycorhizienne s’accompagne de remaniements protéiques dans les organes aériens

Dynamic changes in the subcellular distribution of the tobacco ROS-producing enzyme RBOHD in response to the oomycete elicitor cryptogein.

Highlight text The oomycete elicitor cryptogein triggers the relocation of RBOHD from intracellular compartments to the plasma membrane in tobacco cells. This suggests that intracellular trafficking is a potential determinant of RBOHD activity.

The arbuscular mycorrhizal symbiosis a modulator of cadmium stress

International audience; Ecosystems are submitted to various abiotic stresses, among which heavy metals represent major industrial pollutants. Cadmium (Cd), that has damaging effects on plant metabolism, occurs in agricultural environments through industrial pollution and human activities, including phosphate fertiliser and sewage sludge applications. Metal availability to plants can be modulated by soil microorganisms, such as arbuscular mycorrhizal (AM) fungi. In the present work, Cd effects on the model legume Medicago truncatula inoculated or not with the AM fungus Glomus intraradices have been studied at 3 levels: (1) plant biomass production together with green part chlorophyll quantif…

A proteomic approach to study the effects of two polycyclic aromatic hydrocarbons on arbuscular mycorrhiza of Cichorium intybus roots in gnotobiotic conditions

Proteomes of arbuscular mycorrhizal symbiosis : past and present achievements

Transcription of two blue copper-binding protein isogenes is highly correlated with arbuscular mycorrhizal development in Medicago truncatula.

International audience; Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcp1a and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the mycorrhizal development with Glomus intraradices for up to 7 weeks. Time-course analysis in J5 showed that expression of both MtBcp1 genes increased continuously and correlated strongly with the colonization intensity and arbuscule content. MtPT4, selected as a reference gene of the functional plant-fungus association, showed a weaker correlation to mycorrhizal development. In a second experiment, a range of mycor…

Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?

International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…

Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula

International audience; Because proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and meta…

Asymétrie de la membrane plasmique végétale : caractérisation et rôle dans la signalisation immunitaire des plantes

National audience; La membrane plasmique (MP) forme une barrière sélective entre la cellule et le milieu extérieur.C’est un senseur des modifications environnementales et une plateforme orchestrant latransduction du signal permettant de déployer une réponse adaptée. Elle joue donc un rôleessentiel dans la physiologie cellulaire. Décrypter les bases moléculaires de l’organisation dela MP apparaît donc nécessaire pour comprendre comment les plantes s’adaptent auxmodifications environnementales. La MP est une bicouche de lipides à laquelle des protéinessont associées, et les données accumulées sur ce modèle ont révélé son organisation complexeet dynamique et le rôle essentiel joué par les lipi…

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

International audience; The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing …

Internalization of the a defense elicitor into Nicotiana tabacum cells, cv Bright Yellow 2 (BY-2)