0000000000246490
AUTHOR
L. Konrad
Paternity testing of endangered species of birds by DNA fingerprinting and random amplified polymorphic DNA fingerprinting
Since 1985 DNA fingerprinting is the method of choice to solve forensic problems such as paternity testing (Jeffreys et al., 1986) or identification of immigration cases (Jeffreys et al., 1985 a, b). Its applicability became evident not only in man but also in many biological problems. Jeffreys’probes 33.15 and 33.6 or Epplen’s oligonucleotide probes were used in DNA fingerprinting of dogs and cats (Jeffreys & Morton, 1987), non-human primates (Weiss, 1989), birds (Longmire et al., 1991, Miyaki et al., 1993), fishes (Schartl et al., 1993) or even plants (Weising et al., 1991). Especially its application in paternity testing turned to account in many species of animals (Lubjuhn et al., 1991,…
Bioengineered human bone tissue using autogenous osteoblasts cultured on different biomatrices
Surgical treatment of critical-size posttraumatic bone defects is still a challenging problem, even in modern bone and joint surgery. Progress in cellular and molecular biology during the last decade now permits novel approaches in bone engineering. Recent conceptual and technical advances have enabled the use of mitotically expanded, bone-derived cells as a therapeutic approach for tissue repair. Using three different tissue carrier systems, we successfully cultivated human osteoblasts in a newly developed perfusion chamber. We studied cell proliferation and the expression of osteocalcin, osteopontin, bone morphogenetic protein-2A, alkaline phosphatase, and vascular endothelial growth fact…
The influence of bone allograft processing on osteoblast attachment and function
In order to assess the influence of eight different sterilisation and disinfection methods for bone allografts on adhesion, proliferation, and differentiation of human bone marrow stromal cells (BMSC), cells were grown in culture and then plated onto pieces of human bone allografts. Following processing methods were tested: autoclavation (AUT), low-temperature-plasma sterilisation of demineralised allografts (D-LTP), ethylene oxide sterilisation (EtO), fresh frozen bone (FFB), 80 degrees C-thermodisinfection (80 degrees C), gamma-irradiation (Gamma), chemical solvent disinfection (CSD), and Barrycidal-disinfection (BAR). The seeding efficiency was determined after one hour to detect the num…