Detection of endophytic bacteria in leaves of Vitis vinifera by using fluorescence in situ hybridization

Previous investigation on five cultivars of healthy Sicilian grapevine allowed the isolation of endophytic bacteria belonging to Bacillus genus from different organs (bud, leaf, stalk and shoot). The aim of this work was to use fluorescence in situ hybridization (FISH) experiments in healthy and damaged leaf tissues of Vitis vinifera to visualize and localize bacteria associated with plant materials. The leaves were cleared to minimize the autofluorescence of the plant fragments. The use of fluorescently labelled bacterial probe Eub338 in FISH experiments on discoloured grapevine leaf disks allowed the estimation of the spatial distribution of different bacterial colonies. At the same time,…

Yeast dynamics during a spontaneous white wine fermentation.

Escherichia coli verocitotossici in salsiccia fresca di maiale.