0000000000255972
AUTHOR
Jean-claude Duval
Changes in yield ofin-vivo fluorescence of chlorophyll a as a tool for selective herbicide monitoring
Triazines and derivatives of phenylurea, which are often found in outdoor water samples, induce specific changes in the yield of thein-vivo chlorophyll α-fluorescence of PSII. These changes are correlated quantitatively with the concentration of the herbicides and can therefore be used to set-up a low-price monitor system. In order to detect selectively the herbicide-sensitive part of the fluorescence emission a pulse amplitude modulated fluorimeter was used. The bioassay system was optimised with respect to test organism, growing and measuring conditions. The relationship between fluorescence yield and herbicide concentrations were experimentally determined for the triazines atrazine and s…
Fluorescence induction kinetics as a tool to detect a chlororespiratory activity in the prasinophycean alga, Mantoniella squamata
Abstract Pulse-modulated fluorescence measuring systems were used to study the fluorescence induction kinetics of the primitive prasinophycean alga, Mantoniella squamata . DCMU-treated cells show a very strong non-photochemical quenching which is insensitive to uncouplers and can not be attributed to state transitions or photoinhibition. In order to analyze the origin for this quenching, different inhibitors of the photosynthetic electron flow were applied. It was found that DCCD, an inhibitor of the ATP-synthase as well as of the cytochrome- c oxidase, enhances the fluorescence quenching, whereas antimycin A, which blocks the cyclic electron flow around PS I, stimulates it. The effect of b…