0000000000268218

AUTHOR

Kornél Nagy

showing 3 related works from this author

Analysis of sterols by high-performance liquid chromatography/mass spectrometry combined with chemometrics

2006

A newly developed high-performance liquid chromatography/mass spectrometry (HPLC/MS) method has been successfully used to analyze plasma concentrations of various phytosterols (cholestanol and beta-sitosterol) and cholesterol metabolites (desmosterol and lathosterol). This was based on an unusual solvent combination of water/methanol vs. methanol/acetone/n-hexane applied on a Purospher Star RP-18e (125 x 2 mm, 3 microm) column, which proved excellent for the separation, identification and quantification of plasma sterols. Simple solid-phase extraction preparation of plasma samples was performed, followed by the developed fast and robust HPLC separation. Results on four groups of people were…

Settore MED/09 - Medicina InternaHypercholesterolemiaPilot ProjectsLathosterolMass spectrometryHigh-performance liquid chromatographyMass SpectrometryAnalytical ChemistryChemometricschemistry.chemical_compoundDesmosterolHumansHPLC/MS analysiChromatography High Pressure LiquidSpectroscopyChromatographyCholesterolCholestanolDesmosterolOrganic ChemistryExtraction (chemistry)cholesterol metaboliteDiscriminant AnalysisSitosterolsCholestanolSterolsCholesterolchemistrylipids (amino acids peptides and proteins)HPLCHydroxymethylglutaryl-CoA Reductase InhibitorsRapid Communications in Mass Spectrometry
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Comparative study of serine-plasmalogens in human retina and optic nerve: identification of atypical species with odd carbon chains

2012

International audience; The objective of this work was to detect and identify phosphatidylserine plasmalogen species in human ocular neurons represented by the retina and the optic nerve. Plasmalogens (vinyl-ether bearing phospholipids) are commonly found in the forms of phosphatidylcholine and phosphatidylethanolamine in numerous mammalian cell types, including the retina. While their biological functions are still unclear, the alteration of cellular plasmalogen content has been associated with several human disorders such as Rhizomelic Chondrodysplasia Punctata Type 2 and Primary Open-Angle Glaucoma.By using liquid-chromatography coupled to high resolution and tandem mass spectrometry, we…

MalePlasmalogenPlasmalogensQD415-436PhosphatidylserinesBiologyTandem mass spectrometryBiochemistryRetinaSerine03 medical and health scienceschemistry.chemical_compound0302 clinical medicineEndocrinologySpecies SpecificityTandem Mass Spectrometryphospholipids/phosphatidylserinePhosphatidylcholineSerinemedicineFood and NutritionHumansphospholipidsmass spectrometryAged030304 developmental biologyAged 80 and overchemistry.chemical_classificationPhosphatidylethanolamine0303 health sciencesRhizomelic chondrodysplasia punctataFatty acidOptic NerveCell BiologyPhosphatidylserineEye/retinamedicine.diseaseCarbonchemistryBiochemistryAlimentation et NutritionFemalePatient-Oriented and Epidemiological Research[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition030217 neurology & neurosurgeryChromatography LiquidJournal of Lipid Research
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High Performance Liquid Chromatografy-Mass Spectrometry based chemometric characterization of olive oils

2005

In this study the effective discrimination of extra virgin olive oils is described using HPLC-MS, combined with chemometric evaluation. The presented method is simple since the diluted oil sample is directly injected into the system, without any preliminary chemical derivatization or purification step. Separation of diacylglycerols, triacylglycerols and sterols occurs within 20 min and is achieved using an octadecyl-silica column. Detection is performed by positive APCI mass spectrometry which provided sensitivity to detect over 50 compounds in the sample. After extraction of data, stepwise discriminant function analysis is used to select the variables with the highest discriminative power.…

Linear discriminant analysiAnalytical chemistryAtmospheric-pressure chemical ionizationCross validationMass spectrometrySensitivity and SpecificityBiochemistryHigh-performance liquid chromatographyMass SpectrometryAnalytical ChemistryDiglyceridesChemometricschemistry.chemical_compoundLiquid chromatography–mass spectrometryPlant OilsDerivatizationChromatography High Pressure LiquidTriglyceridesChromatographyOrganic ChemistryOil cultivarDiscriminant AnalysisPhytosterolsReproducibility of ResultsGeneral MedicineLinear discriminant analysisHPLC–MSVegetable oilchemistryOlive oil
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