0000000000274154

AUTHOR

J. Raga

showing 2 related works from this author

Influence of fruit intake and antioxidants on the prevention of opacities of the lens

2008

medicine.medical_specialtyNutrition and Dieteticsbusiness.industryOphthalmologyMedicine (miscellaneous)MedicineFruit intakeLens (geology)businessProceedings of the Nutrition Society
researchProduct

Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay.

1996

Summary Our aim was to evaluate the reverse transcription and polymerase chain reaction (RT/PCR) technique for the detection of rotavirus shedding by infected children as a routine diagnostic procedure, in comparison to the enzyme-linked immunosorbent assay (ELISA), electron microscopy (EM) and polyacrylamide gel etectrophoresis (PAGE) of rotavirus double stranded RNA. Two-hundred and twenty stool specimens were collected from infants and young children with diarrhoea, and 10–20% faecal suspensions were made. Several methods of rotavirus dsRNA extraction were assayed. Electrophoretic analysis of viral RNA was carried out on 10% polyacrylamide gols followed by silver staining. RT/PCR was per…

RotavirusRT/PCRTranscription GeneticImmunologyReoviridaeEnzyme-Linked Immunosorbent AssayMicroscopic électroniquemedicine.disease_causeSensibilité.Polymerase Chain ReactionSensitivity and SpecificityRotavirus InfectionsArticlelaw.inventionFecesfluids and secretionsSensitivitylawVirologyRotavirusViral analysismedicineElectron microscopyHumansTypingChildRotavirus RT/PCRPolymerase chain reactionbiologyRNAInfantbiology.organism_classificationVirologyMolecular biologyReverse transcriptaseVirus SheddingPAGEMicroscopy ElectronReal-time polymerase chain reactionEvaluation Studies as TopicAnalyse viraleRNA ViralElectrophoresis Polyacrylamide GelELISARNA extractionResearch in virology
researchProduct