0000000000276696

AUTHOR

Paula Cristina Brunini Crialesi-legori

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Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae).

2015

Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managi…

media_common.quotation_subjectPopulationBacillus thuringiensisBiologyMothsDiatraea saccharalisCompetition (biology)Lepidoptera genitaliaInsecticide ResistanceHemolysin ProteinsCrambidaeBacterial ProteinsBacillus thuringiensisBotanyAnimalseducationPest Control BiologicalEcology Evolution Behavior and Systematicsmedia_commoneducation.field_of_studyBacillus thuringiensis Toxinsfungifood and beveragesbiology.organism_classificationEndotoxinsCry1AcPEST analysisJournal of invertebrate pathology
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