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RESEARCH PRODUCT

Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae).

Manoel Victor Franco LemosCamila Chiaradia DavolosPatricia Hernández-martínezJanete Apparecida DesidérioPaula Cristina Brunini Crialesi-legoriJuan FerréBaltasar Escriche

subject

media_common.quotation_subjectPopulationBacillus thuringiensisBiologyMothsDiatraea saccharalisCompetition (biology)Lepidoptera genitaliaInsecticide ResistanceHemolysin ProteinsCrambidaeBacterial ProteinsBacillus thuringiensisBotanyAnimalseducationPest Control BiologicalEcology Evolution Behavior and Systematicsmedia_commoneducation.field_of_studyBacillus thuringiensis Toxinsfungifood and beveragesbiology.organism_classificationEndotoxinsCry1AcPEST analysis

description

Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest.

yearjournalcountryeditionlanguage
2015-05-01Journal of invertebrate pathology
10.1016/j.jip.2015.01.013https://pubmed.ncbi.nlm.nih.gov/25736726