Everything you wanted to know about phase and reference frequency in one- and two-dimensional NMR spectroscopy
The fundamental concept of phase discussed in this tutorial aimed at providing students with an explanation of the delays and processing parameters they may find in nuclear magnetic resonance (NMR) pulse programs. We consider the phase of radio-frequency pulses, receiver, and magnetization and how all these parameters are related to phases and offsets of signals in spectra. The impact of the off-resonance effect on the phase of the magnetization is discussed before presenting an overview of how adjustment of the time reference of the free induction decay avoids first-order correction of the phase of spectra. The main objective of this tutorial is to show how the relative phase of a pulse an…
SAN plot: A graphical representation of the signal, noise, and artifacts content of spectra
The signal-to-noise ratio is an important property of NMR spectra. It allows to compare the sensitivity of experiments, the performance of hardware, etc. Its measurement is usually done in a rudimentary manner involving manual operation of selecting separately a region of the spectrum with signal and noise, respectively, applying some operation and returning the signal-to-noise ratio. We introduce here a simple method based on the analysis of the distribution of point intensities in one- and two-dimensional spectra. The signal/artifact/noise plots, (SAN plots) allows one to present in a graphical manner qualitative and quantitative information about spectra. It will be shown that besides me…
Dataset for: Everything you wanted to know about phase and reference frequency in one- and two-dimensional NMR Spectroscopy
The fundamental concept of phase is discussed in this tutorial aimed at providing students with an explanation of the delays and processing parameters they may find in NMR pulse programs. We consider the phase of radio-frequency pulses, receiver, magnetization and how all these parameters are related to phases and offsets of signals in spectra. The impact of the off-resonance effect on the phase of the magnetization is discussed before presenting an overview of how adjustment of the time reference of the free induction decay (FID) avoids first-order correction of the phase of spectra. The main objective of this tutorial is to show how the relative phase of a pulse and the receiver can be us…