0000000000278048
AUTHOR
Ramon Santamaria
MOESM2 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 2: Table S1. Primers used for qRT-PCR.
MOESM1 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 1: Figure S1. Cell sorting strategy using BDFACSAriaIIIâ ˘ cell sorter. Figure S2. Validation of the polycistroniclentiviral vector in HDF.
Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Abstract Background Human fibroblasts can be reprogrammed into induced hepatocyte-like cells through the expression of a set of transcription factors. Although the generation of induced hepatocyte-like cells by HNF4A, HNF1A, and FOXA3 expression has proven to be a robust experimental strategy, using multiple lentivirus results in a highly variable heterogeneous population. Methods We designed and implemented a novel approach based on the delivery of reprogramming factors and green fluorescent protein in a single doxycycline-inducible lentiviral vector using 2A self-cleaving peptides. Results Fibroblasts infected with the lentiviral vector can be amplified in basic fibroblast culture media i…
MOESM1 of Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Additional file 1: Figure S1. Cell sorting strategy using BDFACSAriaIIIâ ˘ cell sorter. Figure S2. Validation of the polycistroniclentiviral vector in HDF.