0000000000278387

AUTHOR

Thierry Magnin

showing 2 related works from this author

Influence of ATPase activity on PPi dependent H+-transport in tonoplast vesicles of Acer pseudoplatanus

1994

Abstract Tonoplast H + -ATPase and H + -pyrophosphatase (H + -PPase) were previously characterized in Acer pseudoplatanus cells (A. Pugin et al., Plant Sci., 73 (1991) 23–34; A. Fraichard et al., Plant Physiol. Biochem., 31 (1993) 349–359). The present study concerns the relationships between these two enzymes in vitro. ATP and PPi hydrolysis were additive and the inhibition of one did not affect the activity of the second one. ATP and PPi H + -transports were also additive. The H + -PPase inhibition did not change ATP-dependent H + -transport but H + -ATPase inhibition inhibited the PPi dependent H + -transport. Because H + -PPase was reported to transport H + and K + into the vacuole (Dav…

0106 biological sciencesTrisStereochemistryATPasePlant ScienceVacuole01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health scienceschemistry.chemical_compoundProton transport[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsTRANSPORT D'IONSGeneticsComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesPyrophosphatasebiologyERABLE FAUX PLATANEGeneral MedicineAcer pseudoplatanusbiology.organism_classificationEnzymechemistryBiochemistrybiology.proteinPMSFAgronomy and Crop Science010606 plant biology & botany
researchProduct

The Tonoplast H+ -ATPase of Acer pseudoplatanus is a vacuolar-type ATPase that operates with a phosphoenzyme intermediate

1995

The tonoplast H+-ATPase of Acer pseudoplatanus has been purified from isolated vacuoles. After solubilization, the purification procedure included size-exclusion and ion-exchange chromatography. The H+-ATPase consists of at least eight subunits, of 95, 66, 56, 54, 40, 38, 31, and 16 kD, that did not cross-react with polyclonal antibodies raised to the plasmalemma ATPase of Arabidopsis thaliana. The 66-kD polypeptide cross-reacted with monoclonal antibodies raised to the 70-kD subunit of the vacuolar H+-ATPase of oat roots. The functional molecular size of the tonoplast H+-ATPase, analyzed in situ by radiation inactivation, was found to be around 400 kD. The 66-kD subunit of the tonoplast H+…

0106 biological sciencesPhysiologyATPaseProtein subunitPlant ScienceVacuole01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health scienceschemistry.chemical_compoundHydroxylamineProton transport[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsGenetics030304 developmental biologychemistry.chemical_classification0303 health sciencesbiologyAcer pseudoplatanusbiology.organism_classificationEnzymechemistryBiochemistryPolyclonal antibodiesbiology.protein010606 plant biology & botanyResearch Article
researchProduct