6533b7d6fe1ef96bd1265e58
RESEARCH PRODUCT
Influence of ATPase activity on PPi dependent H+-transport in tonoplast vesicles of Acer pseudoplatanus
Annick FraichardClaudine TrossatAlain PuginThierry Magninsubject
0106 biological sciencesTrisStereochemistryATPasePlant ScienceVacuole01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health scienceschemistry.chemical_compoundProton transport[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsTRANSPORT D'IONSGeneticsComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesPyrophosphatasebiologyERABLE FAUX PLATANEGeneral MedicineAcer pseudoplatanusbiology.organism_classificationEnzymechemistryBiochemistrybiology.proteinPMSFAgronomy and Crop Science010606 plant biology & botanydescription
Abstract Tonoplast H + -ATPase and H + -pyrophosphatase (H + -PPase) were previously characterized in Acer pseudoplatanus cells (A. Pugin et al., Plant Sci., 73 (1991) 23–34; A. Fraichard et al., Plant Physiol. Biochem., 31 (1993) 349–359). The present study concerns the relationships between these two enzymes in vitro. ATP and PPi hydrolysis were additive and the inhibition of one did not affect the activity of the second one. ATP and PPi H + -transports were also additive. The H + -PPase inhibition did not change ATP-dependent H + -transport but H + -ATPase inhibition inhibited the PPi dependent H + -transport. Because H + -PPase was reported to transport H + and K + into the vacuole (Davies et al., Proc. Natl. Acad. Sci. USA, 89 (1992) 11701–11705), these results led us to suggest that the inhibition of the H + -ATPase activity could modify the H + /K + stoichiometry for the benefit of K + -transport.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-01-01 |