0000000000281230
AUTHOR
Pasquale Alibrandi
Application of PGPB Combined with Variable N Doses Affects Growth, Yield-Related Traits, N-Fertilizer Efficiency and Nutritional Status of Lettuce Grown under Controlled Condition
Nitrogen (N) fertilization is a crucial agricultural practice for boosting production traits in vegetables. However, N synthetic fertilizers—commonly adopted by farmers—have several counterproductive effects on the environment and on humans. The research was performed to assess the combined influence of plant growth promoting bacteria (PGPB) (Azospirillum brasilense DSM 1690, A. brasilense DSM 2298 and Pseudomonas sp. DSM 25356) and various N fertilization doses (0, 30, 60 or 120 kg ha−1) on growth, yield, quality and nitrogen indices of lettuce in protected cultivation. Plant height, root collar diameter, number of leaves and fresh weight were enhanced by A. brasilense DS…
Plant growth promoting potential of bacterial endophytes from three terrestrial mediterranean orchid species
Orchids are highly dependent on symbiotic microorganisms during their entire life cycle. Whereas an important role in orchid seed germination and early plant development is well established for mycorrhizal fungi, the influence of endophytic bacteria on orchid growth has been less investigated. Here, we report the isolation of endophytic bacteria from different organs of three terrestrial Mediterranean orchid species (Spiranthes spiralis, Serapias vomeracea and Neottia ovata), the investigation of their potential Plant Growth-Promoting (PGP) traits and their interaction with the orchid mycorrhizal (OM) fungus Tulasnella calospora in vitro. Little overlap was found among endophytic bacteria i…
The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
AbstractCitrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging to Aspergillus, Quambalaria and Bjerkandera genera—and bacteria—belonging to Staphylococcus genus—were characterized for indoleacetic acid production and phosphate solubilization. Next Generation Sequencing based approaches were then used to characterize the endophytic bacterial and fungal microbiota structures of surface-sterilized C. limon seeds and of shoots obtained under aseptic conditions fr…
The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with potential plant-growth promoting activities
Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methy…