6533b870fe1ef96bd12cfc97
RESEARCH PRODUCT
The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with potential plant-growth promoting activities
Eugenia Mabel GiamminolaGiuseppe GalloMahafizur RahmanSylvia SchnellMarta Leonor De VianaPasquale AlibrandiFrancesco StratiMassimiliano CardinaleMirella CiaccioAnna Maria PugliaPaolo CinàCarlotta De FilippoCarlotta De Filipposubject
0301 basic medicineSeed endophyteFirmicutesFluorescence in situ hybridization–confocal laser scanning microscopy (FISH–CLSM)Beneficial plant–microbe interactionsSeed endophytesSoil SciencePlant ScienceMicrobiology03 medical and health sciencesBotanyColonizationbiologyAlphaproteobacteriafood and beveragesPyrosequencingPlant growth promoting activitiebiology.organism_classificationFluorescence in situ hybridization and confocal laser scanning microscopy (FISH and CLSM)Plant growth promoting activitiesBeneficial plant–microbe interaction030104 developmental biologyBeneficial plant-microbe interactionDelftiaPlant microbiotaMethylobacteriumPyrosequencingAnadenanthera colubrinaSettore BIO/19 - MICROBIOLOGIA GENERALEActinomycesdescription
Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methylobacterium and Staphylococcus spp., exhibiting both plant growth promotion and antimicrobial activities. The two taxonomic groups showed complementary traits, which supports a functional selection. Both genera were detected also by pyrosequencing, together with further taxa. The genera Friedmaniella, Bifidobacterium, Delftia, Anaerococcus and Actinomyces appeared here for the first time as seed endophytes. We detected bacterial cells and micro–colonies in seed cryosections by FISH-CLSM. Alphaproteobacteria, Firmicutes and other bacteria colonized intercellular spaces of the parenchyma and associated to transport vessels. Conclusions: this work sheds light onto the diversity, functions and colonization pattern of the Anadenanthera colubrina seed endophytes, and strongly suggest a role as beneficial partners for seed-associated microbiota.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |