0000000000654550

AUTHOR

Mirella Ciaccio

showing 2 related works from this author

DNA sequence and pattern of expression of the sea urchin (Paracentrotus lividus) alpha-tubulin genes.

1989

To study the molecular aspects of the regulation of transcription of a multigene family, we have isolated and sequenced cDNA and genomic clones coding for the alpha-tubulin of the sea urchin Paracentrotus lividus. Two cDNA clones, P alpha 10 and P alpha 4, contain respectively the coding information for 391 C-terminal and for 338 N-terminal amino acids of the 452 residues that constitute the complete protein. They show silent nucleotide substitutions only, suggesting that P alpha 10 and P alpha 4 represent the cloned copies of two allelic gene transcripts, which encode for two alpha-tubulin isoforms with identical amino acid sequence in the region of the overlap. The comparison of the predi…

Gene isoformSequence analysisMolecular Sequence DataRestriction MappingParacentrotus lividusTranscription (biology)TubulinComplementary DNAGeneticsAnimalsAmino Acid SequenceRNA MessengerPeptide sequenceGeneMammalsbiologyBase SequenceRNACell BiologyDNAbiology.organism_classificationMolecular biologyBiological EvolutionGene Expression RegulationMultigene FamilySea UrchinsDNA ProbesDevelopmental BiologyMolecular reproduction and development
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The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with pot…

2018

Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methy…

0301 basic medicineSeed endophyteFirmicutesFluorescence in situ hybridization–confocal laser scanning microscopy (FISH–CLSM)Beneficial plant–microbe interactionsSeed endophytesSoil SciencePlant ScienceMicrobiology03 medical and health sciencesBotanyColonizationbiologyAlphaproteobacteriafood and beveragesPyrosequencingPlant growth promoting activitiebiology.organism_classificationFluorescence in situ hybridization and confocal laser scanning microscopy (FISH and CLSM)Plant growth promoting activitiesBeneficial plant–microbe interaction030104 developmental biologyBeneficial plant-microbe interactionDelftiaPlant microbiotaMethylobacteriumPyrosequencingAnadenanthera colubrinaSettore BIO/19 - MICROBIOLOGIA GENERALEActinomyces
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