0000000001084928

AUTHOR

Francesco Strati

showing 3 related works from this author

The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures

2021

AbstractCitrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota  have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging to Aspergillus, Quambalaria and Bjerkandera genera—and bacteria—belonging to Staphylococcus genus—were characterized for indoleacetic acid production and phosphate solubilization. Next Generation Sequencing based approaches were then used to characterize the endophytic bacterial and fungal microbiota structures of surface-sterilized C. limon seeds and of shoots obtained under aseptic conditions fr…

0301 basic medicineCitrusScience030106 microbiologyMicrobial communitiesCitrus limonBiologyMicrobiologyArticleApplied microbiology03 medical and health sciencesMicrobial ecologyFISH-CLSMBotanyBjerkanderaRhizosphereMultidisciplinaryEnvironmental microbiologyBacteriaMicrobiotafungiDebaryomycesQFungiRfood and beveragesbiology.organism_classificationseed microbiota bacterial and fungal endophytes surface-sterilized seeds next generation sequencing indoleacetic acid production phosphate solubilization030104 developmental biologyGerminationNGSSeedsShootCitrus limon L. BurmMedicinevertical transmissionPhyllosphereseedPlant ShootsCladosporiumendophytic microbiota
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Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus

2020

AbstractIn the present work, culture-based and culture-independent investigations were performed to determine the microbiota structure of the coelomic fluid of Mediterranean sea urchin Paracentrotus lividus individuals collected from two distinct geographical sites neighboring a high-density population bay and a nature reserve, respectively. Next Generation Sequencing analysis of 16S rRNA gene (rDNA) showed that members of the Proteobacteria, Bacteroidetes and Fusobacteria phyla, which have been previously reported to be commonly retrieved from marine invertebrates, dominate the overall population of microorganisms colonizing this liquid tissue, with minority bacterial genera exhibiting rem…

0301 basic medicineDNA BacterialScience030106 microbiologyPopulationZoologySettore BIO/11 - Biologia MolecolareMicrobial communitiesSettore BIO/19 - Microbiologia GeneraleDNA RibosomalMicrobiologyParacentrotus lividusArticlemicrobiota sea urchin coelomic fluidsea urchin03 medical and health sciencesbiology.animalRNA Ribosomal 16SmicrobiotaAnimalseducationSea urchinPhylogenyeducation.field_of_studyBacteriological TechniquesMultidisciplinarybiologyBacteriaQRBacteroidetesHigh-Throughput Nucleotide SequencingFusobacteriaMarine invertebratesSequence Analysis DNAbiology.organism_classificationcoelomic fuid030104 developmental biologyEchinodermParacentrotus lividusParacentrotusMedicineProteobacteria
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The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with pot…

2018

Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methy…

0301 basic medicineSeed endophyteFirmicutesFluorescence in situ hybridization–confocal laser scanning microscopy (FISH–CLSM)Beneficial plant–microbe interactionsSeed endophytesSoil SciencePlant ScienceMicrobiology03 medical and health sciencesBotanyColonizationbiologyAlphaproteobacteriafood and beveragesPyrosequencingPlant growth promoting activitiebiology.organism_classificationFluorescence in situ hybridization and confocal laser scanning microscopy (FISH and CLSM)Plant growth promoting activitiesBeneficial plant–microbe interaction030104 developmental biologyBeneficial plant-microbe interactionDelftiaPlant microbiotaMethylobacteriumPyrosequencingAnadenanthera colubrinaSettore BIO/19 - MICROBIOLOGIA GENERALEActinomyces
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