0000000000281476

AUTHOR

Valentina Failla

showing 3 related works from this author

PFN1 and integrin‐β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells

2019

Abstract Ex vivo limbal stem cell transplantation is the main therapeutic approach to address a complete and functional re‐epithelialization in corneal blindness, the second most common eye disorder. Although important key points were defined, the molecular mechanisms involved in the epithelial phenotype determination are unclear. Our previous studies have demonstrated the pluripotency and immune‐modulatory of fibroblast limbal stem cells (f‐LSCs), isolated from the corneal limbus. We defined a proteomic profile especially enriched in wound healing and cytoskeleton‐remodelling proteins, including Profilin‐1 (PFN1). In this study we postulate that pfn‐1 knock down promotes epithelial lineage…

0301 basic medicinelimbal stem cellApoptosisintegrin-β1Settore MED/13 - EndocrinologiaProfilins0302 clinical medicinesignallingCells CulturedCorneal epitheliumIntegrin beta1TOR Serine-Threonine KinasesEpithelium CornealCell DifferentiationCell biologymedicine.anatomical_structuremTOR pathway030220 oncology & carcinogenesisMolecular MedicineOriginal ArticleStem cellHomeobox protein NANOGintegrin‐β1regenerative medicineBiologyLimbus CorneaeCorneal limbus03 medical and health sciencesstem cellsmedicineHumansprofilinFibroblastlimbal stem cellsPI3K/AKT/mTOR pathwayCell ProliferationWound HealingSettore MED/30 - Malattie Apparato VisivoCell BiologyOriginal ArticlesFibroblastseye diseasesepithelial differentiation030104 developmental biologyGene Expression RegulationEye disordersense organscorneal regenerationWound healingBiomarkersJournal of Cellular and Molecular Medicine
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Anti-Inflammatory Action of Heterogeneous Nuclear Ribonucleoprotein A2/B1 in Patients with Autoimmune Endocrine Disorders

2019

Our previous studies documented that human fibroblast-limbal stem cells (f-LSCs) possess immunosuppressive capabilities, playing a role in regulating T-cell activity. This study highlights the molecular activities by which human f-LSCs can attenuate the inflammatory responses of self-reactive peripheral blood mononuclear cells (PBMCs) collected from patients with autoimmune endocrine diseases (AEDs). Anti-CD3 activated PBMCs from twenty healthy donors and fifty-two patients with AEDs were cocultured on f-LSC monolayer. 2D-DIGE proteomic experiments, mass spectrometry sequencing and functional in vitro assays were assessed in cocultured PBMCs. We identified the downmodulation of several huma…

Gene isoformInflammationfibroblast-limbal stem cells Autoimmune Endocrine DiseaseNF-ĸB interaction.Peripheral blood mononuclear cellArticleSettore MED/13 - EndocrinologiaAutoimmune Endocrine Diseases03 medical and health sciencesNF-ĸB interaction0302 clinical medicinemedicineGene silencingIL-2 receptorSettore BIO/06 - Anatomia Comparata E CitologiaHeterogeneous Nuclear Ribonucleoprotein A2/B1hnRNP A2/B1030304 developmental biology0303 health sciencesSettore MED/30 - Malattie Apparato Visivobusiness.industryautoimmunityGeneral Medicinefibroblast-limbal stem cellsSettore BIO/18 - Genetica030220 oncology & carcinogenesisCancer researchfibroblast-limbal stem cells Autoimmune Endocrine Diseasesmedicine.symptomStem cellbusinessCD8immunotoleranceJournal of Clinical Medicine
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Bilateral Ultrathin Descemet’s Stripping Automated Endothelial Keratoplasty vs. Bilateral Penetrating Keratoplasty in Fuchs’ Dystrophy: Corneal Highe…

2021

Background and Objectives: The objective of this paper is to compare the visual outcomes and quality of life (QoL) after bilateral ultrathin Descemet’s stripping automated endothelial keratoplasty (UT-DSAEK) with bilateral penetrating keratoplasty (PK) for Fuchs’ endothelial dystrophy (FED). Materials and Methods: Retrospective comparative cohort study, including 11 patients with FED who underwent bilateral PK and 13 patients with FED who underwent bilateral UT-DSAEK. All patients were already pseudophakic or had undergone a combined cataract procedure. The main outcomes were corrected distance visual acuity (CDVA) corneal higher-order aberrations (HOAs), contrast sensitivity (CS) and quali…

medicine.medical_specialtyMedicine (General)Visual acuityDistance visual acuitygenetic structuresvisual acuitymedia_common.quotation_subjectmedicine.medical_treatmentFuchs' dystrophyArticleCohort StudiesR5-920Quality of lifeOphthalmologyhigher-order aberrationmedicineContrast (vision)HumansEye surgerymedia_commonRetrospective Studiescontrast sensitivityhigher-order aberrationsbusiness.industryFuchs' Endothelial DystrophyDystrophybilateral penetrating keratoplastyGeneral Medicinemedicine.diseasebilateral ultrathin DSAEKeye diseasesAberrations of the eyebilateral ultrathin DSAEK; bilateral penetrating keratoplasty; visual acuity; contrast sensitivity; higher-order aberrations; quality of lifequality of lifevisual acuity.medicine.symptombusinessDescemet Stripping Endothelial KeratoplastyKeratoplasty PenetratingMedicina
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