0000000000282853
AUTHOR
Rolf Lauterbach
Energy Transfer between Surface-Immobilized Light-Harvesting Chlorophyll a/b Complex (LHCII) Studied by Surface Plasmon Field-Enhanced Fluorescence Spectroscopy (SPFS)
The major light-harvesting chlorophyll a/b complex (LHCII) of the photosynthetic apparatus in green plants can be viewed as a protein scaffold binding and positioning a large number of pigment molecules that combines rapid and efficient excitation energy transfer with effective protection of its pigments from photobleaching. These properties make LHCII potentially interesting as a light harvester (or a model thereof) in photoelectronic applications. Most of such applications would require the LHCII to be immobilized on a solid surface. In a previous study we showed the immobilization of recombinant LHCII on functionalized gold surfaces via a 6-histidine tag (His tag) in the protein moiety. …
Chapter 3 Surface Plasmon Optics for the Characterization of Biofunctional Architectures
Publisher Summary This chapter describes a wide variety of work employing surface plasmon resonance (SPR), surface plasmon field-enhanced fluorescence spectroscopy (SPFS), and surface plasmon field-enhanced diffraction spectroscopy (SPDS) analysis, specifically for investigations relevant to biofunctional, bio-affinity aspects. The SPR has shown already its impact on interfacial analysis. The combination of these three methods has the potential to generate more information and reveal details of biofunctional interfaces. The versatility of the SPR technique is shown by a vast amount of publications in the past decades; the method has matured into a well-accepted analytical tool for the chara…