0000000000285296

AUTHOR

Nikolai Lebovka

New approaches for the effective valorization of papaya seeds: Extraction of proteins, phenolic compounds, carbohydrates, and isothiocyanates assisted by pulsed electric energy

Abstract The study compares the efficiency of common aqueous extraction (CE) at different pH (2.5–11) and temperatures (20–60 °C) and extraction assisted by pulsed electric energy (pulsed electric fields, PEF or high voltage electrical discharges, HVED) of nutritionally valuable and antioxidant compounds from papaya seeds. The exponential decay pulses with initial electric field strengths of ≈ 13.3 kV/cm and ≈ 40 kV/cm for PEF and HVED treatments, respectively, were used. The number of pulses n was changed within 1–2000. The impacts of temperature and pH on extraction efficiency of different components (proteins, total phenolic compounds, carbohydrates, isothiocyanates) and antioxidant capa…

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Ultrasound-assisted green solvent extraction of high-added value compounds from microalgae Nannochloropsis spp.

Abstract The aim of this work was to investigate ultrasound (US)-assisted green solvent extraction of valuable compounds from the microalgae Nannochloropsis spp. Individual green solvents (water, ethanol (EtOH), dimethyl sulfoxide (DMSO)) and binary mixture of solvents (water-DMSO and water-EtOH) were used for the extraction procedures. Maximum total phenolic compounds yield ( Y p  ≈ 0.33) was obtained after US pre-treatment ( W  = 400 W, 15 min), being almost 5-folds higher compared to that found for the untreated samples and aqueous extraction ( Y p  ≈ 0.06). The highest yield of total chlorophylls ( Y c  ≈ 0.043) was obtained after US ( W  = 400 W, 7.5 min), being more than 9-folds highe…

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Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis

Abstract The study was aimed at investigation of the potential of pulsed electric field (PEF) pre-treatment as a preliminary step of pH-assisted aqueous extraction of algae components from microalgae Nannochloropsis suspensions. The PEF and sonication (S) were compared as pretreatment methods. They were applied at normal (pH = 8.5) and basic (pH = 11) conditions, and supplementary basic extraction (at pH = 11) was done. The extracts were analyzed for content of pigments, proteins, carbohydrates, total phenolic compounds and antioxidant capacity. The colloidal stability of PEF- and S-pretreated suspensions was also evaluated. The data evidence that PEF technique allows selective extraction o…

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“Ice” juice from apples obtained by pressing at subzero temperatures of apples pretreated by pulsed electric fields

Abstract The impact of apple pretreatment by pulsed electric field (PEF) on juice extraction using the freezing-assisted pressing was studied. Apple discs were PEF pretreated at electric field strength of E  = 800 V/cm and then air blast frozen inside the freezer (− 40 °C). Then, pressing experiments in a laboratory-pressing chamber (2–5 bars) were started at sub-zero temperature (− 5 °C). Time evolution of juice yield and its nutritional qualities were compared for PEF and untreated apple samples. High improvements of juice yield were obtained for freeze-thawed (FT) and PEF + FT samples. The combination of PEF + pressing (5 bar) at sub-zero temperature gave optimum results for juice extrac…

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Impact of pulsed electric fields and high voltage electrical discharges on extraction of high-added value compounds from papaya peels

Abstract Extraction of the nutritionally valuable and antioxidant compounds from papaya peels using conventional aqueous extraction (E) (at 20 °C, 50 °C, and 60 °C) and extraction assisted by pulsed electric field (PEF) or by high voltage electrical discharges (HVED) was studied. Increase of extraction temperature or extraction in basic media (pH = 11) not always resulted in an increase of the yields or TEAC values. E.g., the concentration of proteins and TEAC values at pH = 7 and pH = 11 were noticeably smaller at 60 °C as compared to those measured at 50 °C. HVED-assisted technique showed a higher extraction efficiency of high-added value compounds compared to PEF-assisted extraction. E.g…

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Electron spin resonance as a tool to monitor the influence of novel processing technologies on food properties

Abstract Background Nowadays, electron spin resonance (ESR) is widely used as a powerful, non-destructive and very sensitive technique for the detection of free radicals in food systems. It can be applied for the direct identification of highly reactive oxygen species, organic and inorganic paramagnetic species and screening of food for potential toxicity. Its applications cover investigating food oxidative stability and properties of irradiated foods including fruits and vegetables, meats and fishes, spices, cereal grains, and oil seeds. Scope and approach This review aims at providing specialists in food science and industry with the fundamentals of ESR spectroscopy, typical radicals pres…

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Pulsed electric field assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organic solvents and water

Abstract This work studies the potential of the pulsed electric field (PEF) assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organic solvents (dimethyl sulfoxide, DMSO and ethanol, EtOH) and water. The one-stage (E I ) and two stage (E II ) extraction procedures were compared. The procedure E I included the common extraction using the binary mixtures. Two stage (E II ) extraction procedure involved PEF-treatment (20 kV/cm) of microalgae suspension (1% wt.) and extraction in water as the first step. The second step included the common extraction using the binary mixtures. The effects of extraction procedure and concentra…

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Current applications and new opportunities for the use of pulsed electric fields in food science and industry

ABSTRACT Several studies have demonstrated the feasibility of pulsed electric fields (PEF) for different applications in food industry. PEF technology is therefore a valuable tool that can improve functionality, extractability, and recovery of nutritionally valuable compounds as well as the bioavailability of micronutrients and components in a diverse variety of foods. Additionally, other studies have shown the potential of PEF treatments to reduce food processing contaminants and pesticides. This opens the doors to new PEF applications in the food industry. This review focused on some of the most renowned traditional and emerging PEF applications for improvement of osmotic dehydration, ext…

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Electro-biorefinery as a Potential Tool for Valorization of Mango and Papaya By-products

During exotic fruits processing, a large amount of by-products, especially seeds and peels, are produced and discarded into the environment causing organic pollution. However, it is recognized that these by-products are a good source of high-added value compounds, which can be used for different purposes (i.e. food additives and/or nutraceuticals). At this stage of development, there is a lack of information about the different extraction methods that can be used for the recovery of high-added value compounds from papaya and mango by-products. In this line, the extraction assisted by pulsed electric technologies seems to be rather promising. In this research study, the exponential decay pul…

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