0000000000287625
AUTHOR
Sandrine Lepiller
showing 3 related works from this author
Comparative analysis of zebrafish nos2a and nos2b genes
2008
Abstract Nitric oxide synthase (NOS) produces nitric oxide (NO) from arginine. Three NOS isoforms have been identified in mammals, namely a neuronal (NOS1), an inducible (NOS2) and an endothelial (NOS3) enzyme. In zebrafish genome, one nos1 gene and two nos2 genes (nos2a and nos2b) were observed. We cloned zebrafish nos2a cDNA and compared nos2a and nos2b sequences, expression and inducibility. When analyzed by reverse transcription-PCR, the expression of nos2a remained very low during initial development, then increased at 96 hpf, while nos2b was expressed from 6 hpf and subsequently remained stable. Expression of nos2a is detected in the head, eye and gut regions by WISH experiments perfo…
Imaging of nitric oxide in a living vertebrate using a diaminofluorescein probe
2007
Abstract Numerous approaches have been described to identify nitric oxide (NO), a free radical involved in various physiological and pathophysiological processes. One of these approaches is based on the use of chemical probes whose transformation by NO generates highly fluorescent derivatives, permitting detection of NO down to nanomolar concentrations. Here, we show that the cell-permeant diaminofluorophore 4-amino-5-methylamino-2′-7′-difluorofluorescein diacetate (DAF-FM-DA) can be used to detect NO production sites in a living vertebrate, the zebrafish Danio rerio. The staining pattern obtained in larvae includes the bulbus arteriosus, forming bones, the notochord, and the caudal fin. Th…
Corrigendum to “Imaging of nitric oxide in a living vertebrate using a diaminofluorescein probe” [Free Radic. Biol. Med. 43 (2007) 619–627]
2008
Adrian Grimes and colleagues showed in a previous report that zebrafish bulbus arteriosus and the smooth muscle component of the chick cardiac outflow tract may be specifically labeled by DAF-2DA. Using this fluorescent dye they could distinguish the zebrafish bulbus arteriosus from "true" cardiac chambers, the atrium and ventricle (Grimes AC, Stadt HA, Shepherd IT, Kirby ML. Solving an enigma: arterial pole development in the zebrafish heart. Dev Biol 2006 Feb 15;290(2):265−76). The authors regret not including this information in the original version of their article.