0000000000289975
AUTHOR
Mikus Gavars
Seroģenētiskās izmeklēšanas loma celiakijas diagnostikā
Darbā gaitā izvērtēta kombinētas seroloģiskās un ģenētiskās secīgas izmeklēšanas loma celiakijas diagnostikā, kā arī noteikta celiakijas sastopamība Latvijas populācijā, ņemot vērā seroloģisko un ģenētisko izmeklējumu rezultātus. Metodes: Celiakijai raksturīgās antivielas tika noteiktas 1443 pacientiem. 43 seropozitīvajiem veikta arī celiakijas genotipa noteikšana. Aptuveni piecas reizes vairāk seronegatīvajiem paraugiem arī tika noteikts HLA-DQ genotips. Rezultāti: Ar seroģenētisko metodi noteiktā celiakijas prevalence Latvijas populācijā ir no 0,28% līdz 1,25%. Rezultāti atkarīgi no aprēķinos izmantotajiem kritērijiem, taču visprecīzāk noteiktā ir 0,42%.Viltus pozitīvi seroloģisko testu r…
First Report on the Latvian SARS-CoV-2 Isolate Genetic Diversity
Remaining a major healthcare concern with nearly 29 million confirmed cases worldwide at the time of writing, novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 920 thousand deaths since its outbreak in China, December 2019. First case of a person testing positive for SARS-CoV-2 infection within the territory of the Republic of Latvia was registered on 2nd of March 2020, 9 days prior to the pandemic declaration by WHO. Since then, more than 277,000 tests were carried out confirming a total of 1,464 cases of coronavirus disease 2019 (COVID-19) in the country as of 12th of September 2020. Rapidly reacting to the spread of the infection, an ongoing sequenci…
Pathogenic Escherichia coli Among Asymptomatic Children and Associated Factors
Abstract Although Escherichia coli is a part of the commensal flora of the gastrointestinal tract, pathogenic types of E. coli can cause diarrhea, especially in children. Pathogenic types are found also in healthy individuals, but prevalence of pathogenic E. coli among asymptomatic children varies and has not been studied in Latvia. The aim of the study was to determine prevalence of pathogenic E. coli among asymptomatic children and identify factors associated with presence of bacterium. Children (aged 0.5–8 years) without acute gastrointestinal symptoms were included in a cross-sectional study. Parents were asked to answer a questionnaire (demographic data, parental education, type of del…
Nestriktu intervālu metode optimālā ražošanas plāna noteikšanai nenoteikta pieprasījuma gadījumā
Darbā apskatīta nestriktu intervālu metode optimālā ražošanas plāna noteikšanai nenoteiktu pieprasījumu gadījumā. Galvenais uzsvars likts uz optimālā ražošanas plāna modeļa izstrādi, izmantojot nestriktus intervālus un skaitļus, lai varētu izmantot klasisko matemātiskās programmēšanas tehniku optimālā atrisinājuma atrašanai atbilstoši vienam vai vairākiem optimizācijas kritērijiem. Metode ir ilustrēta ar skaitliskiem piemēriem.
Prevalence estimation of celiac disease in the general adult population of Latvia using serology and HLA genotyping
Background: Prevalence estimates for celiac disease (CD) depend on the method used. The role of deamidated gliadin peptide (DGP) and genetic testing in epidemiological studies and diagnostic settings of celiac disease (CD) has still to be established. Objectives: The objective of this article is to assess the prevalence of CD in Latvia by combining serological tests with DQ2.5/ DQ8 testing. Methods: A total of 1444 adults from a randomly selected cross-sectional general population sample were tested by ELISA for tTG IgA, DGP IgA and IgG antibodies (QUANTA Lite, Inova Diagnostics Inc). Samples with tTG IgA 20U were tested for EMA IgA by indirect immunofluorescence assay, and all specimens wi…
Saliva as a testing sample for SARS-CoV-2 detection by RT-PCR in low prevalence community settings
AbstractObjectivesThe number of COVID-19 cases is increasing globally and there is an urgency for a simple non-invasive method for the detection of SARS-CoV-2. Our study aimed to demonstrate that saliva can be used as a specimen for SARS-CoV-2 detection notably for the screening of extensive population groups via pooling.MethodsTo demonstrate that saliva is an appropriate specimen for SARS-CoV-2 detection a field study including 3,660 participants was performed between September 29 and October 1, 2020. We collected paired nasopharyngeal/oropharyngeal swabs (NPS) and saliva specimens and processed them within 24 hours of collection. We performed 36 serial measurements of 8 SARS-CoV-2 positiv…