Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture

Background/Aim: Epithelial–mesenchymal transition (EMT) is a process co-opted by cancer cells to invade and form metastases. In the present study we analyzed gene expression profiles of primary breast cancer cells in culture in order to highlight genes related to EMT. Materials and Methods: Microarray expression analysis of primary cells isolated from a specimen of a patient with an infiltrating ductal carcinoma of the breast was performed. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) analyses validated microarray gene expression trends. Results: Thirty-six candidate genes were selected and used to generate a molecular network displaying the tight relationship among them. The …

CLINICAL, BIOMOLECULAR AND IMMUNOLOGICAL INVESTIGATION OF KNEE OSTEOARTHRITIS

Introduction. Osteoarthritis (OA) is a slowly progressive disorder characterized by a gradual loss of articular cartilage. The OA classification is mainly based on clinical and radiographic evaluations, using the Knee Society Score (AKSS) and the Kellgren and Lawrence (KL) scale. Single nucleotide polymorphisms (SNPs) in frzb, matn3,aspn, pthr2, gdf5 and dvwa genes are involved in OA susceptibility. However, very few studies in Caucasian people describe the association between KL grading scale and genetical alterations. In this study we assessed the associations between KL grade, clinical features,SNPs and inflammatory mediators in Sicilian individuals Methods. We enrolled 66 patients with …

Theoretical and experimental study of the kinetics of particle chains near electrodes in dielectrophoretic devices

Using a three dimensional coupled Monte Carlo-Poisson algorithm and experimental results we studied the role of the particle-particle dipole interaction on the kinetics of a system of human cells suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. We found that the kinetics of the cells during negative/repulsive dielectrophoresis depends on the local distribution of particles. If the cells have generated long chains during positive/attractive dielectrophoresis, such chains can hinder subsequent detachment resulting in a reduction of the separation/manipulation efficiency of dielectrophoretic device. This effec…

Curcumin delivered by solid lipid nanoparticles potentiates radiation effects in MCF7 cancer cells

Inflammatory Response to a High Radiation Dose in Breast Cancer

Background: Radiation therapy (RT) is an essential treatment modality used for breast cancer (BC) care. Radiations can stimulate the immune system via the early activation of cytokine cascades, which can greatly affect cellular radio-sensitivity. Our aim is to analyze inflammatory response induced by high ionizing radiation (IR) doses, generated by intraoperative radiotherapy (IORT) treatment, to identify several potential targets that may influence cell radio-response. Methods: MCF10, MCF7 and MDA-MB231 BC cell lines have been exposed to high IR (23 Gray and 9 Gray), delivered by IORT treatments. Conditioned media (CM) were assayed at the time points: 0’, 30’, 1, 3, 6, 24, 48, and 72 hours…