Asthmatic Bronchial Epithelial Cells (BEC) do not undergo apoptosis in response to Rhinovirus (RV) infection and this is associated with increased viral replication.

Effect of IL-13Ralpha2 Receptor levels on the biological activity of IL-13 variant R110Q.

Medium-Term Culture of Primary Oral Squamous Cell Carcinoma in a Three-Dimensional Model: Effects on Cell Survival Following Topical 5-Fluororacile Delivery by Drug-Loaded Matrix Tablets

Since the activity of several conventional anticancer drugs is restricted by resistance mechanisms and dose-limiting side-effects, the design of formulations for local application on malignant lesions seems to be an efficient and promising drug delivery approach. In this study, the effect of locally applied 5-FU on cell death was evaluated both in a SCC4/HEK001 model and in a newly proposed 3D outgrowth model of oral squamous cell carcinoma (OSCC). Initially, the optimal drug dose was established by delivery of solutions containing different amounts of 5-FU. The solution containing 1% (w/v) of 5-FU resulted effective in inducing cell death with complete eradication of cell colonies. Buccal …

The temporal expression of ciliated bronchial epithelium (CBE) 1 in embryonic lungs.

Studying embrionic lung development by tissue engineering.

Asthmatic bronchial epithelial cells have a deficient innate immune response to infection with rhinovirus.

Effects of antioxidants on CSE-induced cell death in human asthmatic primary bronchial epithelial cells

The link between cigarette smoke (CS) and lung inflammation is quite strong, however relatively little is still known on the effects of CS on human bronchial epithelial cells survival during asthma. In this study we focused our attention on the apoptotic effects of CS on healthy (HC) and asthmatic (AS) primary bronchial epithelial cells (PBEC) and on the role of antioxidants to protect epithelial cells from CSE-induced apoptosis. Twenty subjects (10 HC and 10 AS) were recruited for this study and PBEC were obtained by bronchoscopy. PBEC were treated with oxidants (H2O), anti-oxidants (GSH and AA) and cigarette smoke extracts (CSE). Early apoptosis (EA) and necrosis were measured by flow cyt…

Inflammatory mediators release from asthmatic & non-asthmatic bronchial epithelial cells following infection with rhinovirus (RV)

Characterization of a novel three-dimensional outgrowth model of human bronchial mucosa

Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obt…