Modelling of the elution behaviour in hybrid micellar eluents with different organic modifiers

Abstract The elution behaviour in reversed-phase liquid chromatography (RPLC), with mobile phases containing micelles, can be described according to a distribution pattern among three phases: bulk water, micellar pseudophase and stationary phase. Several mechanistic equations based on this pattern, previously used to predict the retention in hybrid mobile phases of sodium dodecyl sulphate and propanol, were checked with several organic modifiers and a group of solutes of diverse polarity. The modifiers were the alcohols 1-propanol, 1-butanol and 1-pentanol, and other organic solvents widely used in conventional RPLC such as acetonitrile and tetrahydrofuran. New models were also proposed to …