0000000000291514
AUTHOR
Bruno Frohlich
showing 3 related works from this author
Minimally invasive bone biopsies of fully wrapped mummies guided by computed tomography and fibre-optic endoscopy: Methods and suggested guidelines
2020
Abstract Recent advances in the recovery and analysis of ancient DNA (aDNA) and application of isotopic analysis of tissue obtained from mummified human remains has been accompanied by continued advances in non-invasive imaging using X-ray computed tomography (CT) and use of minimally invasive surgical techniques employing small fibre-optic endoscopes. We used these state-of-the-art techniques to examine ancient Egyptian mummies in the Egyptian Museum and Papyrus Collection of Berlin, obtaining bone samples for aDNA, stable isotope analyses and radiocarbon dating. CT and endoscopic guidance were applied to locate and biopsy bones using pre-existing access points in order to avoid any furthe…
The efficacy of whole human genome capture on ancient dental calculus and dentin
2019
Objectives Dental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques. Materials and methods Total DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in-solution hybridization capture and high-throughput sequencing. Results Total DNA from calculus exceeded that of dentin in all cases, and altho…
Differential preservation of endogenous human and microbial DNA in dental calculus and dentin.
2018
AbstractDental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is microb…