0000000000291525
AUTHOR
Wilfried Rosendahl
Minimally invasive bone biopsies of fully wrapped mummies guided by computed tomography and fibre-optic endoscopy: Methods and suggested guidelines
Abstract Recent advances in the recovery and analysis of ancient DNA (aDNA) and application of isotopic analysis of tissue obtained from mummified human remains has been accompanied by continued advances in non-invasive imaging using X-ray computed tomography (CT) and use of minimally invasive surgical techniques employing small fibre-optic endoscopes. We used these state-of-the-art techniques to examine ancient Egyptian mummies in the Egyptian Museum and Papyrus Collection of Berlin, obtaining bone samples for aDNA, stable isotope analyses and radiocarbon dating. CT and endoscopic guidance were applied to locate and biopsy bones using pre-existing access points in order to avoid any furthe…
Blunt force trauma: an exceptional example of an ancient Egyptian mummy head
In the course of a scientific cooperation between the German Mummy Project at the Reiss-Engelhorn-Museen, Mannheim (Germany) and the Musée National d'Histoire et d'Art Luxembourg (Luxembourg), an ancient Egyptian mummy head was analyzed using a multidisciplinary approach including radiocarbon dating, ultra-high resolution computed tomography, physical anthropology, forensic medicine and Egyptology. Dated to the Roman Period, the mummy head belonged to an upper-class woman between 25 and 35 years of age. Computed tomography revealed a lethal blunt force trauma affecting the dorsal parts of the parietal bones, below the intact overlaying soft tissue. Moreover, ancient medical treatment was ev…
Molecular phylogeny of the extinct cave lion Panthera leo spelaea.
To reconstruct the phylogenetic position of the extinct cave lion (Panthera leo spelaea), we sequenced 1 kb of the mitochondrial cytochrome b gene from two Pleistocene cave lion DNA samples (47 and 32 ky B.P.). Phylogenetic analysis shows that the ancient sequences form a clade that is most closely related to the extant lions from Africa and Asia; at the same time, cave lions appear to be highly distinct from their living relatives. Our data show that these cave lion sequences represent lineages that were isolated from lions in Africa and Asia since their dispersal over Europe about 600 ky B.P., as they are not found among our sample of extant populations. The cave lion lineages presented h…
Neanderthals and Monkeys in the Würmian of Central Europe: The Middle Paleolithic Site of Hunas, Southern Germany
The site of Hunas is a cave ruin, filled with bedded sediments up to the roof. About 20 m sediments from the top down were excavated and yielded Middle Paleolithic artifacts as well as numerous faunal remains, including Macaca. With a single human molar, the site is one of the rare Neanderthalian localities in Germany. New TIMS-U/Th dating of speleothems at the base of the profile indicate that the whole sequence was not deposited during the late Middle Pleistocene as previously thought, but during the last glacial. According to the new chronological results, Hunas is the only place which shows the coexistence of man and monkey in the Wurmian of Central Europe. The Macaca remains are the mo…
Phylogeography of lions (Panthera leossp.) reveals three distinct taxa and a late Pleistocene reduction in genetic diversity
Lions were the most widespread carnivores in the late Pleistocene, ranging from southern Africa to the southern USA, but little is known about the evolutionary relationships among these Pleistocene populations or the dynamics that led to their extinction. Using ancient DNA techniques, we obtained mitochondrial sequences from 52 individuals sampled across the present and former range of lions. Phylogenetic analysis revealed three distinct clusters: (i) modern lions, Panthera leo; (ii) extinct Pleistocene cave lions, which formed a homogeneous population extending from Europe across Beringia (Siberia, Alaska and western Canada); and (iii) extinct American lions, which formed a separate popula…