Die stark wachsende chemische Vielfalt der RNA-Modifikationen enthält eine Thioacetalstruktur

Inside Cover: A Vastly Increased Chemical Variety of RNA Modifications Containing a Thioacetal Structure (Angew. Chem. Int. Ed. 26/2018)

Innentitelbild: Die stark wachsende chemische Vielfalt der RNA-Modifikationen enthält eine Thioacetalstruktur (Angew. Chem. 26/2018)

A Vastly Increased Chemical Variety of RNA Modifications Containing a Thioacetal Structure

International audience; Recently discovered new chemical entities in RNA modifications have involved surprising functional groups that enlarge the chemical space of RNA. Using LC-MS, we found over 100 signals of RNA constituents that contained a ribose moiety in tRNAs from E. coli. Feeding experiments with variegated stable isotope labeled compounds identified 37 compounds that are new structures of RNA modifications. One structure was elucidated by deuterium exchange and high-resolution mass spectrometry. The structure of msms2 i6 A (2-methylthiomethylenethio-N6-isopentenyl-adenosine) was confirmed by methione-D3 feeding experiments and by synthesis of the nucleobase. The msms2 i6 A contai…

A protein-RNA interaction atlas of the ribosome biogenesis factor AATF

AbstractAATF is a central regulator of the cellular outcome upon p53 activation, a finding that has primarily been attributed to its function as a transcription factor. Recent data showed that AATF is essential for ribosome biogenesis and plays a role in rRNA maturation. AATF has been implicated to fulfil this role through direct interaction with rRNA and was identified in several RNA-interactome capture experiments. Here, we provide a first comprehensive analysis of the RNA bound by AATF using CLIP-sequencing. Interestingly, this approach shows predominant binding of the 45S pre-ribosomal RNA precursor molecules. Furthermore, AATF binds to mRNAs encoding for ribosome biogenesis factors as …