Vertical stratification and trophic interactions among organisms of a soil decomposer food web – a field experiment using 15N as a tool

Abstract In this field study, we explored the spatial segregation between the litter- and humus-inhabiting organisms of the detrital food web using 15 N-isotope technique. The study was established in 11 × 11 m plots fertilized with 15 N-labelled urea. Ten years after urea application, soil samples were taken, both from the litter layer and the combined F+H layer. The samples were analysed for N content and the proportion of 15 N in (i) the residual organic matter in the litter and F+H layer (excluding microbes), (ii) microbial biomass, and (iii) various feeding guilds of soil fauna. The basal resource, soil microbes, and the fauna were more enriched with 15 N in the F+H layer than in the l…