0000000000291914
AUTHOR
Adriana Fandiño
Age‐induced change in the color of the human crystalline lens
Purpose To determine the chromatic coordinates of different human crystalline lenses to analyze the age-induced change in the color of the crystalline. Methods The spectral transmission of nine human crystalline lenses of different ages were measured by using a Perkin-Elmer 800 UV/VIS spectrophotometer. Using these data we calculated the chromatic coordinates of each crystalline lens for both solar and incandescent illumination. The study adheres to the tenets of the Declaration of Helsinki for Research Including Human Subjects and was approved by the Institutional Review Board. Results The results show a greater saturation of color with the increase of age. In principle, the change tends t…
Spectral Transmission of the Human Crystalline Lens in Adult and Elderly Persons: Color and Total Transmission of Visible Light
Purpose To experimentally measure the spectral transmission of human crystalline lenses belonging to adult and elderly persons, and to determine the color and total transmission of visible light of such crystalline lenses. Methods The spectral transmission curve of 32 human crystalline lenses was measured using a PerkinElmer 800UV/VIS spectrometer. Total transmission of visible light and the chromatic coordinates of these crystalline lenses were determined from these curves for solar illumination. Results The crystalline lens that filters UV and its transmission in the visible spectrum decreases with age; such a decrease is greater for short wavelengths. The total transmission of visible li…
Light transmission of the human lens and its relation with aging
Purpose To determine the visible light transmission of the human crystalline lens of persons of different ages, using both solar and incandescent illumination. Methods The spectral transmission of lens was measured by using the Perkin-Elmer 800 UV/VIS spectrophotometer. With these data we calculated the percentage of visible light transmission for solar illumination, simulated by the CIE D65 illuminant, and for incandescent illumination (A illuminant). Results The total amount of visible light transmitted by nine crystalline lenses is represented as a function of age: with solar illumination and with incandescent illumination and the comparison between both illuminants.In general, the amoun…
The influence of storage method on the transparency of pig crystalline lens
Este artículo se ha publicado de forma definitiva en: http://www.scielo.br/pdf/abo/v82n1/0004-2749-abo-82-01-0056.pdf Purpose: The porcine eye is frequently used as a research model. This paper analyzes the effect of different storage methods on the transparency of pig crystalline lens. Methods: A spectral transmission curve (from 220 to 780 nm) for the crystalline lens was determined experimentally after storage in different conditions: saline solution, formalin, castor oil, and freezing at -80°C. The total transmission in the visible spectrum, which was used as an index of transparency, was calculated from these curves. For comparative purposes, fresh lenses were evaluated and used as con…
The influence of storage method on the transparency of pig crystalline lens
ABSTRACT Purpose: The porcine eye is frequently used as a research model. This paper analyzes the effect of different storage methods on the transparency of pig crystalline lens. Methods: A spectral transmission curve (from 220 to 780 nm) for the crystalline lens was determined experimentally after storage in different conditions: saline solution, formalin, castor oil, and freezing at -80°C. The total transmission in the visible spectrum, which was used as an index of transparency, was calculated from these curves. For comparative purposes, fresh lenses were evaluated and used as controls. Results: Storing the porcine crystalline lens in saline solution or castor oil resulted in a transpare…