0000000000294004
AUTHOR
Rossella Calabrese
SCAFFOLD A STRUTTURA POLISACCARIDICA/POLIAMINOACIDICA PER IL RILASCIO SOSTENUTO DI DNA
Scaffolds of Hyaluronan crosslinked with alpha,beta-polyaspartylhydrazide for uses in tissue engineering field
Hydrogels for potential colon drug release by thiol-ene conjugate addition of a new inulin derivative.
Inulin was chosen as a starting polymer for biocompatible, pH-sensitive and biodegradable hydrogels. Three INUDVSA-TT hydrogels were obtained by crosslinking inulin derivatives with trimethylolpropane tris(3-mercaptopropionate) under varying conditions. The resulting hydrogels were cell compatible, as demonstrated by MTS and trypan blue exclusion assays acting on Caco-2 cells, and were biodegraded by inulinase and esterase, thus suggesting their use as colonic drug delivery systems. 2-Methoxyestradiol, an anti-cancer drug, was soaked in INUDVSA-TT hydrogels and its in vitro release and apoptotic effect on Caco-2 cells were evaluated.
New injectable depot systems based on alpha,beta-poly(N-2-hydroxyethyl)-D,L-aspartammide copolymers
Sintesi di nuovi copolimeri graft di acido ialuronico,polietilenglicole ed acido lattico
SINTESI E CARATTERIZZAZIONE DI NUOVI FILM POLIMERICI A BASE DI ACIDO IALURONICO E DI UN DERIVATO ETILENDIAMMINICO DEL PHEA PER LA PREVENZIONE DELLE ADESIONI POST-OPERATORIE
HYALURONAN/POLYASPARTAMIDE BASED BIOMATERIALS AS GENE ACTIVATED MATRICES
HYALURONAN CROSS-LINKED WITH alpha,beta-POLYASPARTYLHYDRAZIDE BASED SCAFFOLDS FOR TISSUE ENGINEERING
A NOVEL POLYSACCHARIDE/POLYAMINOACID pH-SENSITIVE AND BIODEGRADABLE HYDROGEL: PREPARATION AND CHARACTERIZATION
SYNTHESIS OF NOVEL GRAFT COPOLYMERS OF HYALURONAN, POLYETHYLENEGLYCOL AND POLYLACTIC ACID
CROSSLINKED HYALURONAN WITH A PROTEIN-LIKE POLYMER: NOVEL FILMS FOR BIOMEDICAL APPLICATION
Biodegradable and pH-sensitive hydrogels for potential colon-specific drug delivery: Characterization and in vitro release studies
A novel pH-sensitive and biodegradable composite hydrogel, based on a methacrylated and succinic derivative of dextran, named Dex-MA-SA, and a methacrylated and succinic derivative of alpha,beta-poly( N-2-hydroxyethyl)- DL-aspartamide (PHEA), named PHM-SA, was produced by photocross-linking. The goal was to obtain a colon-specific drug delivery system, exploiting both the pH-sensitive behavior and the colon-specific degradability. The hydrogel prepared with a suitable ratio between the polysaccharide and the polyaminoacid was characterized regarding its swelling behavior in gastrointestinal simulated conditions, chemical and enzymatic degradability, interaction with mucin, and cell compatib…
COMPOSITE BIOMATERIALS OF HYALURONIC ACID AND A PROTEIN-LIKE POLYMER FOR TISSUE ENGINEERING
Polysaccharide/polyaminoacid composite scaffolds for modified DNA release.
Abstract In this work composite polymeric films or sponges, based on hyaluronic acid (HA) covalently crosslinked with α,β-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)- d , l -aspartamide (PE), have been prepared and characterized as local gene delivery systems. In particular, HA/PE scaffolds have been loaded with PE/DNA interpolyelectrolyte complexes, employing PE as a macromolecular crosslinker for HA and as a non-viral vector for DNA. In vitro studies showed that HA/PE films and sponges have high compatibility with human dermal fibroblasts and they give a sustained DNA release, whose trend can be easily tailored by varying the crosslinking ratio between HA and PE. Electrophoresis analysi…
CROSSLINKED HYALURONAN WITH A PROTEIN-LIKE POLYMER: NOVEL BIORESORBABLE FILMS FOR BIOMEDICAL APPLICATIONS
In this work, novel hydrogel films based on hyaluronan (HA) chemically crosslinked with the alpha,beta-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) were produced by solution casting method. The goal was to exploit both the biological key role of HA in tissue repair and regeneration, and the versatility of a synthetic protein-like polymer as the PHEA-EDA, in order to obtain biomaterials with physicochemical and biological properties suitable for a clinical use. By varying the molar ratio between the PHEA-EDA amino groups and HA carboxyl groups, three different films were obtained and characterized. Particularly FTIR, swelling, hydrolysis, and enzymatic degradatio…
COMPOSITE SCAFFOLD FOR TISSUE ENGINEERING
NUOVI FILM A STRUTTURA POLISACCARIDICA E POLIAMMINOACIDICA PER LA PREVENZIONE DELLE ADESIONI POST-CHIRURGICHE
FLUORINATED DERIVATIVES OF A POLYASPARTAMIDE BEARING POLYETHYLENE GLYCOL CHAINS AS OXYGEN CARRIERS
Abstract In this paper the synthesis and characterization of new fluorinated polymers based on a polyaspartamide bearing polyethylene glycol (PEG) chains, are reported. The starting material was the α,β-poly(N-2-hydroxyethyl)- dl -aspartamide (PHEA), a water soluble and biocompatible polymer, that has been derivatized with both polyethylene glycol (with a molecular weight of 2000 Da) and 5-pentafluorophenyl-3-perfluoroheptyl-1,2,4-oxadiazole. By varying the amount of the fluorinated oxadiazole, three samples have been prepared and characterized by FT-IR, 1H NMR, 19F NMR and UV–VIS spectroscopy. Size exclusion chromatography analysis of these copolymers revealed the occurrence of a self-asso…