6533b850fe1ef96bd12a8620

RESEARCH PRODUCT

CROSSLINKED HYALURONAN WITH A PROTEIN-LIKE POLYMER: NOVEL BIORESORBABLE FILMS FOR BIOMEDICAL APPLICATIONS

Emanuela Fabiola CraparoRossella CalabreseGiovanna PitarresiGaetano GiammonaFabio Salvatore Palumbo

subject

Materials scienceCell SurvivalBiomedical EngineeringTetrazolium SaltsBiomaterialsHydrolysischemistry.chemical_compoundTissue engineeringCoated Materials BiocompatibleHyaluronidaseNephelometry and TurbidimetryPolymer chemistryHyaluronic acidSpectroscopy Fourier Transform InfraredmedicineCell AdhesionPolyaminesHumansHyaluronic AcidAspartameSkinchemistry.chemical_classificationWound HealingHydrolysisMetals and AlloysProteinsCalorimetry IndirectHydrogelsPolymerTrypan BlueFibroblastsThiazolesCross-Linking ReagentschemistryChemical engineeringHYDROGELS HYALURONIC ACIDSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogelsCeramics and CompositesTrypan blueSwellingmedicine.symptommedicine.drug

description

In this work, novel hydrogel films based on hyaluronan (HA) chemically crosslinked with the alpha,beta-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) were produced by solution casting method. The goal was to exploit both the biological key role of HA in tissue repair and regeneration, and the versatility of a synthetic protein-like polymer as the PHEA-EDA, in order to obtain biomaterials with physicochemical and biological properties suitable for a clinical use. By varying the molar ratio between the PHEA-EDA amino groups and HA carboxyl groups, three different films were obtained and characterized. Particularly FTIR, swelling, hydrolysis, and enzymatic degradation studies were performed. In addition, the cytocompatibility of HA/PHEA-EDA hydrogel films was evaluated using human derm fibroblasts, by means of MTT and trypan blue exclusion assays. The high swelling capability, the long-term hydrolysis resistance, and the resistance to hyaluronidase greater than that of only HA, together with the cell compatibility, have suggested the potential application of these novel HA-based hydrogel films in the biomedical field of tissue engineering.

yearjournalcountryeditionlanguage
2007-07-06
10.1002/jbm.a.31316http://hdl.handle.net/10447/37866