0000000000294772

AUTHOR

Jürgen Winkler

showing 3 related works from this author

Sevoflurane affects neurogenesis after forebrain ischemia in rats.

2007

BACKGROUND: The effect of sevoflurane on the neuroregenerative potential after neuronal injury is unclear. We investigated the effect of low and high concentrations of sevoflurane on endogenous neurogenesis after cerebral ischemia. METHODS: Anesthetized and ventilated rats were randomized to four different treatment groups. Groups 1 and 2: 1.4% sevoflurane; Groups 3 and 4: 2.8% sevoflurane. In Groups 1 and 3, no cerebral ischemia was induced (sham-operated). In Groups 2 and 4, 10 min of forebrain ischemia was induced by bilateral carotid artery occlusion plus hemorrhagic hypotension. Physiological variables were maintained constant. Bromodeoxyuridine was given as a marker of neurogenesis. A…

MaleMethyl EthersIschemiaH&E stainHippocampusHippocampusSevofluraneBrain IschemiaRats Sprague-Dawleychemistry.chemical_compoundRandom AllocationSevofluraneProsencephalonmedicineAnimalsCell ProliferationNeuronsDose-Response Relationship Drugbusiness.industryDentate gyrusNeurogenesismedicine.diseaseNerve RegenerationRatsDose–response relationshipDisease Models AnimalAnesthesiology and Pain MedicinechemistryAnesthesiaAnesthetics InhalationDentate GyrusbusinessBromodeoxyuridinemedicine.drugAnesthesia and analgesia
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Neuronal precursor-specific activity of a human doublecortin regulatory sequence.

2005

The doublecortin (DCX) gene encodes a 40-kDa microtubule-associated protein specifically expressed in neuronal precursors of the developing and adult CNS. Due to its specific expression pattern, attention was drawn to DCX as a marker for neuronal precursors and neurogenesis, thereby underscoring the importance of its promoter identification and promoter analysis. Here, we analysed the human DCX regulatory sequence and confined it to a 3.5-kb fragment upstream of the ATG start codon. We demonstrate by transient transfection experiments that this fragment is sufficient and specific to drive expression of reporter genes in embryonic and adult neuronal precursors. The activity of this regulator…

Doublecortin Domain ProteinsDoublecortin Protein5' Flanking RegionBlotting WesternMolecular Sequence DataRegulatory Sequences Nucleic AcidTransfectionBiochemistryHippocampusCellular and Molecular NeuroscienceMiceGene expressionAnimalsHumansCell LineageGrowth SubstancesGeneTranscription factorCells CulturedSequence DeletionRegulation of gene expressionNeuronsReporter genebiologyBase SequenceStem CellsNeurogenesisNeuropeptidesBrainSequence Analysis DNAMolecular biologyDoublecortinMice Inbred C57BLGene Expression RegulationRegulatory sequencebiology.proteinMicrotubule-Associated ProteinsJournal of neurochemistry
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Human SPG11 cerebral organoids reveal cortical neurogenesis impairment

2018

Abstract Spastic paraplegia gene 11(SPG11)-linked hereditary spastic paraplegia is a complex monogenic neurodegenerative disease that in addition to spastic paraplegia is characterized by childhood onset cognitive impairment, thin corpus callosum and enlarged ventricles. We have previously shown impaired proliferation of SPG11 neural progenitor cells (NPCs). For the delineation of potential defect in SPG11 brain development we employ 2D culture systems and 3D human brain organoids derived from SPG11 patients’ iPSC and controls. We reveal that an increased rate of asymmetric divisions of NPCs leads to proliferation defect, causing premature neurogenesis. Correspondingly, SPG11 organoids appe…

GenotypeHereditary spastic paraplegiaNeurogenesisFluorescent Antibody TechniqueBiology03 medical and health sciencesGlycogen Synthase Kinase 3GeneticsOrganoidmedicineSpasticHumansMolecular BiologyGenetics (clinical)Allelesbeta CateninCerebral Cortex0303 health sciences030305 genetics & heredityNeurogenesisProteinsGeneral MedicineHuman brainmedicine.diseaseNeural stem cellnervous system diseasesOrganoidsmedicine.anatomical_structurePhenotypeMutationGeneral ArticleDisease SusceptibilityParaplegiaCognition DisordersNeuroscienceNeural developmentBiomarkersHuman Molecular Genetics
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