0000000000297273
AUTHOR
Michael G. Ikonomou
Comparison of Three Techniques for Lipid Removal from Seal Blubber: Gel Permeation, Acid Treatment, and Dialysis with Semipermeable Membrane
Abstract Harbor seal blubber samples were analyzed for PCDD/Fs and PCBs by splitting the initial extract into three aliquots and applying three different techniques for lipid removal (the first step of sample cleanup methodology for GC-HRMS analysis): gel permeation chromatography (GPC), sulfuric acid treatment, and dialysis through semipermeable membrane. Correlation coefficients of analyte concentrations obtained from three sets of replicate samples ranged from 0.965 to 0.994. In addition, a number of seal blubber samples were processed without pre-extraction using only the dialysis technique. The analyte concentrations in these samples correlated well with the analyte concentrations obta…
Uptake rates of semipermeable membrane devices (SPMDs) for PCDDs, PCDFs and PCBs in water and sediment.
Uptake rates of several PCDDs, PCDFs and PCBs were measured for semipermeable membrane devices (SPMDs) under controlled conditions in bulk water and sediment. The study was performed at 19 degrees C and 11 degrees C, and water and sediment concentrations were measured during the exposure. Linear uptake rates for specific PCDD/Fs and PCBs in 19 degrees C water varied from 34 to 111 l/m2 day and in 11 degrees C water from 8.8 to 96 l/m2 day for the whole SPMD. Uptake rates at 19 degrees C sediment ranged from 9.0 to 80 mgOC/m2 day and in 11 degrees C sediment, from 3.0 to 31, mgOC/m2 day. Partitioning of the compounds between membrane and lipid was also measured during the linear uptake phase…
Lipid-containing semipermeable membrane devices (SPMDs) as concentrators of toxic chemicals in the lower Fraser River, Vancouver, British Columbia.
Abstract Semipermeable membrane devices (SPMDs) were deployed in the water column and in the sediments of the Lower Fraser River (B.C., Canada) to compare the levels and the congener profiles of polychlorinated dibenzo- p -dioxins (PCDDs), dibenzofurans (PCDFs) and non- ortho -chlorinated biphenyls (N-o,o′-PCBs) against those sampled by an Infiltrex resin column water sampler. Also the relationship between what was present in the ecosystem as sampled by SPMDs and what was accumulated in the tissue of resident benthic-feeding fish was explored. Only the lipid portion of the SPMDs was prepared for analysis, due to some technical difficulties in cleaning the polyethene tubing. The calculated w…