Experimental and natural cathodoluminescence in the shell of Crassostrea gigas from Thau lagoon (France): ecological and environmental implications.

We present a cathodoluminescence (CL) study of growth layer deposition in the shell of the oyster Crassostrea gigas. CL is based on the physical properties of lattice-bound manganese (Mn2+), which is the main activator in calcium carbonate. Our study involved chemical marking by immersing individuals in seawater to which manganese chloride had been added, and subsequent reading of the shell with CL microscopy coupled with numeric treatment of microphotographs; CL emission was analyzed using a scanning electron microscope coupled to a spectrometer. Since the marking did not harm the oysters, repeated markings were possible, allowing validation of the inferences made from analysis of the shel…