0000000000302588
AUTHOR
Jean-jacques Bessoule
Les indicateurs de l'état biologique des sols : Guide de sélection selon le type d’application et/ou la problématique
National audience
Lipid Rafts in Higher Plant Cells
A large body of evidence from the past decade supports the existence of functional microdomains in membranes of animal and yeast cells, which play important roles in protein sorting, signal transduction, or infection by pathogens. They are based on the dynamic clustering of sphingolipids and cholesterol or ergosterol and are characterized by their insolubility, at low temperature, in nonionic detergents. Here we show that similar microdomains also exist in plant plasma membrane isolated from both tobacco leaves and BY2 cells. Tobacco lipid rafts were found to be greatly enriched in a sphingolipid, identified as glycosylceramide, as well as in a mixture of stigmasterol, sitosterol, 24-methyl…
Soil bioindicators: how to select them in function of the site issues?
Soil bioindicators: how to select them in function of the site issues?. 13th International AquaConSoil Conference
Application of soil bioindicators for risk assessment, monitoring and soil characterization in contaminated soils. Results from the French national “Bioindicators Programme”
International audience; absent
Application of soil bioindicators for risk assessment, monitoring and soil characterization in contamined soils. Results from the French national "Bioindicators Programme"
EA GenoSol IPM CT?; International audience
Involvement of Lipoxygenase-dependent Production of Fatty Acid Hydroperoxides in the Development of the Hypersensitive Cell Death induced by Cryptogein on Tobacco Leaves
Lipid peroxidation was investigated in relation with the hypersensitive reaction in cryptogein-elicited tobacco leaves. A massive production of free polyunsaturated fatty acid (PUFA) hydroperoxides dependent on a 9-lipoxygenase (LOX) activity was characterized during the development of leaf necrosis. The process occurred after a lag phase of 12 h, was accompanied by the concomitant increase of 9-LOX activity, and preceded by a transient accumulation of LOX transcripts. Free radical-mediated lipid peroxidation represented 10% of the process. Inhibition and activation of the LOX pathway was shown to inhibit or to activate cell death, and evidence was provided that fatty acid hydroperoxides ar…