0000000000304107

AUTHOR

Mã©lanie Lemunier

showing 2 related works from this author

Distribution and diversity of type III secretion system-like genes in saprophytic and phytopathogenic fluorescent pseudomonads

2004

Type three secretion systems (TTSSs) are protein translocation mechanisms associated with bacterial pathogenicity in host plants, and hypersensitive reactions in non-host plants. Distribution and diversity of TTSS-like genes within a collection of saprophytic and phytopathogenic fluorescent pseudomonads were characterized. This collection included 16 strains belonging to 13 pathogenic species, and 87 strains belonging to five saprophytic species isolated from plant rhizosphere and soil. Presence of conserved hypersensitive reaction/pathogenicity (hrp) genes (hrc RST) was assessed both by PCR using primers designed to amplify the corresponding sequence and by dot-blot hybridization using a P…

DNA BacterialMolecular Sequence DataBiologyPlant RootsPolymerase Chain ReactionApplied Microbiology and BiotechnologyMicrobiologyFluorescenceMicrobiologyType three secretion systemlaw.inventionPSEUDOMOMAS FLUORESCENS03 medical and health sciencesBacterial ProteinslawPseudomonasRNA Ribosomal 16SGenotypeGene[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologySoil MicrobiologyPolymerase chain reactionComputingMilieux_MISCELLANEOUSPlant Diseases030304 developmental biology2. Zero hungerGenetics0303 health sciencesEcology030306 microbiologyGenetic transferGenetic VariationSequence Analysis DNAPlants16S ribosomal RNAbiology.organism_classification[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyPOUVOIR PATHOGENERestriction fragment length polymorphismPolymorphism Restriction Fragment LengthBacteria
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Conservation of type III secretion system genes inBradyrhizobiumisolated from soybean

2006

International audience; The distribution of rhcRST genes encoding the type III secretion system (T3SS) in a collection of Bradyrhizobium strains was characterized by PCR and Southern blot hybridization. The polymorphism of the corresponding sequences amplified by PCR was characterized by RFLP and sequencing together with those available in the databank. Genomic group I is characterized by the presence of Bradyrhizobium elkanii strains and group II by the presence of B. japonicum and B. liaoningense strains. Highly conserved T3SS-like genes were detected by PCR in all Bradyrhizobium strains isolated from soybean belonging to genomic group II, and in none of the strains belonging to genomic g…

DNA BacterialGenotyperhc genessinorhizobiumhrc genesMicrobiologyBradyrhizobiummicroorganisme du sollaw.invention03 medical and health scienceslawGeneticsRELATION PLANTE-MICROORGANISMESymbiosisMolecular BiologyGenePhylogenyBradyrhizobium elkaniiPolymerase chain reaction030304 developmental biologySouthern blotGenetics0303 health sciencesBase Sequencebradyrhizobiumbiologymesorhizobium030306 microbiologyGenetic transferbiochemical phenomena metabolism and nutritionRibosomal RNAbiology.organism_classificationtype III secretion system-T3SSRNA BacterialPhenotype[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyGenes BacterialRNA RibosomalbacteriaSoybeansRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthFEMS Microbiology Letters
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