0000000000308260
AUTHOR
M. Grube
Stillage as a Source of Growth Promoting Biofactors and a Stimulator of Levan and Extracellular Levansucrase Synthesis for Zymomonas mobilis
In the present work, the fermentation of simultaneous production of ethanol and levan by Zymomonas mobilis grown on different growth media has been studied. Yeast extract, rye stillage or sugar beet molasses stillage were used as additives to the basic sucrose media and the chemical composition, including vitamins, of the cultivation liquids have been determined. It has been shown that 0.5 % of yeast extract dry weight additive could be substituted by 10.0 % of native stillage additive. It was established that molasses stillage stimulates the ethanol synthesis, but rye stillage additive is more preferable for levan production. The extracellular levansucrase obtained from the culture liquid …
Formally described species woefully underrepresent phylogenetic diversity in the common lichen photobiont genus Trebouxia (Trebouxiophyceae, Chlorophyta): An impetus for developing an integrated taxonomy.
Lichens provide valuable systems for studying symbiotic interactions. In lichens, these interactions are frequently described in terms of availability, selectivity and specificity of the mycobionts and photobionts towards one another. The lichen-forming, green algal genus Trebouxia Puymaly is among the most widespread photobiont, associating with a broad range of lichen-forming fungi. To date, 29 species have been described, but studies consistently indicate that the vast majority of species-level lineages still lack formal description, and new, previously unrecognized lineages are frequently reported. To reappraise the diversity and the evolutionary relationships of species-level lineages …
Carbohydrates in Jerusalem artichoke powder suspension
Purpose – The aim of this study was to evaluate the influence of temperature and action time on the extraction rate of carbohydrates of Jerusalem artichoke concentrate powder and inactivation of inulin during boiling and sterilization.Design/methodology/approach – Water suspension of Jerusalem artichoke concentrate (5g/100ml) at 25, 50 and 100○C was tested after 5, 15, 30 and 60min to determine the content of inulin, glucose, fructose and sucrose and evaluate the extraction rate. The stability of inulin was studied after boiling and sterilization at 120○C during 1, 2 and 3h. The extraction rate was evaluated by Fourier‐Transform Infrared (FT‐IR) spectroscopy as well.Findings – It was shown …
PO-224 Fourier transform infrared spectroscopy-based detection of hypoxia-induced biomolecular changes in different cancer cell lines
Introduction Our previous studies have shown that under hypoxic stress human colorectal and breast cancer cells produce higher amounts of lipids compared to that under normoxic environment. The aim of this study was to estimate hypoxia induced cell metabolic response via the biochemical composition in prostate and lung cancer cells by using Fourier transform infrared spectroscopy (FTIR). Material and methods Lung cancer cell lines NCI-H69, COR-L23 and prostate cancer cell line PC3, and primary prostate cancer culture NFI-56 were seed in serum-free medium in density 1 × 106 per well in triplicates in 6-well plates and incubated for 48 in normoxic and hypoxic (5% CO2, 1% O2 and 94% N2) condit…