0000000000319769
AUTHOR
G. Nagel
Global age-sex-specific fertility, mortality, healthy life expectancy (HALE), and population estimates in 204 countries and territories, 1950–2019: a comprehensive demographic analysis for the Global Burden of Disease Study 2019
Background Accurate and up-to-date assessment of demographic metrics is crucial for understanding a wide range of social, economic, and public health issues that affect populations worldwide. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 produced updated and comprehensive demographic assessments of the key indicators of fertility, mortality, migration, and population for 204 countries and territories and selected subnational locations from 1950 to 2019. Methods 8078 country-years of vital registration and sample registration data, 938 surveys, 349 censuses, and 238 other sources were identified and used to estimate age-specific fertility. Spatiotemporal Gaussian…
460. MGMTP140K Vector Constructs for Efficient In Vivo Enrichment of Genetically Corrected Cells Following Hematopoietic Cell Gene Transfer
MGMTP140K, a mutant of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) resistant to the inhibitor O6-benzylguanine (BG), protects hematopoietic stem cells (HSC) from the toxicity of alkylating agents such as temozolomide (TMZ) and, therefore, allows efficient in vivo enrichment of transduced cells by BG/TMZ therapy. MGMTP140K has been proposed as a selection marker in gene therapy of monogeneic diseases. If used for this purpose, however, MGMTP140K expression in vectors containing internal ribosomal entry sites (IRES) would be from the compromised 3' position, as the 5' position will be required for the therapeutic gene. Thus, we have investigated SFFV/MESV-based vector…
Five insights from the Global Burden of Disease Study 2019
The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 provides a rules-based synthesis of the available evidence on levels and trends in health outcomes, a diverse set of risk factors, and health system responses. GBD 2019 covered 204 countries and territories, as well as first administrative level disaggregations for 22 countries, from 1990 to 2019. Because GBD is highly standardised and comprehensive, spanning both fatal and non-fatal outcomes, and uses a mutually exclusive and collectively exhaustive list of hierarchical disease and injury causes, the study provides a powerful basis for detailed and broad insights on global health trends and emerging challenges. GBD …