Modulation of free-flight response in Drosophila

Incipient speciation in Drosophila melanogaster involves chemical signals.

WOS: 000300572900001; International audience; The sensory and genetic bases of incipient speciation between strains of Drosophila melanogaster from Zimbabwe and those from elsewhere are unknown. We studied mating behaviour between eight strains - six from Zimbabwe, together with two cosmopolitan strains. The Zimbabwe strains showed significant sexual isolation when paired with cosmopolitan males, due to Zimbabwe females discriminating against these males. Our results show that flies' cuticular hydrocarbons (CHs) were involved in this sexual isolation, but that visual and acoustic signals were not. The mating frequency of Zimbabwe females was highly significantly negatively correlated with t…

Desat expression in neural and non-neural tissues separately affects perception and emission of sex pheromones in Drosophila

The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster

Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — in Drosophila melanogaster. In p...

Gene Regulation and Species-Specific Evolution of Free Flight Odor Tracking in Drosophila

Running title: flight evolution in Drosophila This is an invited contribution to the special issue on Genetics of Adaptation based on a symposium of the same name at the National Centre for Biological Sciences (TIFR, Bangalore, India) in November 2016; International audience; The flying ability of insects has coevolved with the development of organs necessary to take-off from the ground, generate, and modulate lift during flight in complex environments. Flight orientation to the appropriate food source and mating partner depends on the perception and integration of multiple chemical signals. We used a wind tunnel-based assay to investigate the natural and molecular evolution of free flight …

desat1 and the Evolution of Pheromonal Communication in Drosophila

1749-6632 (Electronic) 0077-8923; The evolution of communication is a fundamental biological problem. The genetic control of the signal and its reception must be tightly coadapted, especially in interindividual sexual communication. However, there is very little experimental evidence for tight genetic linkage connecting the emission of a signal and its reception. In Drosophila melanogaster, desat1 is the first known gene that simultaneously affects the emission and the perception of sex pheromones. Our experiments show that both aspects of pheromonal communication (the emission and the perception of sex pheromones) depend on distinct genetic control and may result from tissue-specific expre…

Effect of genes, social experience, and their interaction on the courtship behaviour of transgenic Drosophila males

Behaviour depends (a) on genes that specify the neural and non-neural elements involved in the perception of and responses to sensory stimuli and (b) on experience that can modulate the fine development of these elements. We exposed transgenic and control Drosophila melanogaster males, and their hybrids, to male siblings during adult development and measured the contribution of genes and of experience to their courtship behaviour. The transgene CheB42a specifically targets male gustatory sensillae and alters the perception of male inhibitory pheromones which leads to frequent male–male interactions. The age at which social experience occurred and the genotype of tester males induced a varia…

Experimental introgression to evaluate the impact of sex specific traits onDrosophila melanogasterincipient speciation

ABSTRACTSex specific traits are involved in speciation but it is difficult to determine whether their variation initiates or reinforces sexual isolation. In some insects, speciation depends of the rapid change of expression in desaturase genes coding for sex pheromones. Two closely related desaturase genes are involved inDrosophila melanogasterpheromonal communication:desat1affects both the production and the reception of sex pheromones whiledesat2is involved in their production in flies of Zimbabwe populations. There is a strong asymmetric sexual isolation between Zimbabwe populations and all other “Cosmopolitan” populations: Zimbabwe females rarely copulate with Cosmopolitan males whereas…

The consequences of regulation of desat1 expression for pheromone emission and detection in Drosophila melanogaster.

AbstractSensory communication depends on the precise matching between the emission and the perception of sex- and species-specific signals; understanding both the coevolutionary process and the genes involved in both production and detection is a major challenge. desat1 determines both aspects of communication—a mutation in desat1 simultaneously alters both sex pheromone emission and perception in Drosophila melanogaster flies. We investigated whether the alteration of pheromonal perception is a consequence of the altered production of pheromones or if the two phenotypes are independently controlled by the same locus. Using several genetic tools, we were able to separately manipulate the tw…

Genes involved in sex pheromone discrimination in Drosophila melanogaster and their background-dependent effect.

International audience; Mate choice is based on the comparison of the sensory quality of potential mating partners, and sex pheromones play an important role in this process. In Drosophila melanogaster, contact pheromones differ between male and female in their content and in their effects on male courtship, both inhibitory and stimulatory. To investigate the genetic basis of sex pheromone discrimination, we experimentally selected males showing either a higher or lower ability to discriminate sex pheromones over 20 generations. This experimental selection was carried out in parallel on two different genetic backgrounds: wild-type and desat1 mutant, in which parental males showed high and l…

Genes involved in sex pheromones discrimination and their context-dependent effect

Mate choice is based on the comparison of the sensory quality of potential mating partners and sex pheromones are important with regard to mate choice. In Drosophila melanogaster, male and female contact pheromones diverge both for their occurence and for their effects — respectively inhibitory and stimulatory — on male courtship. To investigate the genetic basis of sex pheromones discrimination in male flies, we experimentally selected, during 20 generations, lines in which males showed either the higher or the lower ability to discriminate sex pheromones. This experimental selection was carried out with wild-type and desat1 mutant genetic backgrounds in which parental males respectively s…

Chemically mediated sexual isolation between Zimbabwean endemic and cosmopolitan populations of Drosophila melanogaster

Chemically mediated sexual isolation between Zimbabwean endemic and cosmopolitan populations of [i]Drosophila melanogaster[/i]. ISCE 26. annual meeting

Genes involved in sex pheromone discrimination and their context-dependent effect

International audience

Revisited Roles of Drosophila Female Pheromones

All tests involved a pair of 5-day-old male and female (intact or decapitated) flies. Females were ‘homotypic’ (same species and strain as the tested male: D. melanogaster, Cs strain; D. mauritiana, 163.1 strain; D. simulans, Seychelles strain), ‘desat1 non-perfumed’ (D. melanogaster desat1 mutant), ‘perfumed’ (desat1 with transfer of Cs females pheromones), or ‘Cs’ (D. melanogaster control strain). Data shown are the frequencies of courtship (with both intact and decapitated females) and of mating (with intact females), within a 1 h observation period and were calculated from the total number of tested pairs (shown in brackets). D. mauritiana males courted (χ2 = 16.81, P < 0.001) and mated…

Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation

AbstractSex specific traits are involved in speciation but it is difficult to determine whether their variation initiates or reinforces sexual isolation. In some insects, speciation depends of the rapid change of expression in desaturase genes coding for sex pheromones. Two closely related desaturase genes are involved in Drosophila melanogaster pheromonal communication: desat1 affects both the production and the reception of sex pheromones while desat2 is involved in their production in flies of Zimbabwe populations. There is a strong asymmetric sexual isolation between Zimbabwe populations and all other “Cosmopolitan” populations: Zimbabwe females rarely copulate with Cosmopolitan males w…

Antennal lobe representations are optimized when olfactory stimuli are periodically structured to simulate natural wing beat effects

Animals use behaviors to actively sample the environment across a broad spectrum of sensory domains. These behaviors discretize the sensory experience into unique spatiotemporal moments, minimize sensory adaptation, and enhance perception. In olfaction, behaviors such as sniffing, antennal flicking, and wing beating all act to periodically expose olfactory epithelium. In mammals, it is thought that sniffing enhances neural representations; however, the effects of insect wing beating on representations remain unknown. To determine how well the antennal lobe produces odor-dependent representations when wing beating effects are simulated, we used extracellular methods to record neural units an…

The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster

Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — in Drosophila melanogaster. In particular, desat1 is necessary for the biosynthesis of major cuticular pheromones in both males and females. It is also involved in the male ability to discriminate sex pheromones. Each of these two sensory communication aspects depends on distinct desat1 putative regulatory regions. Here, we used (i) mutant alleles resulting from the insertion/excision of a transposable genomic element inserted in a desat1 regulatory region, and (ii) transgenics made with desat1 regulatory regions used to target …

Supplemental Material for Cortot et al., 2019

The file "Cortot-SupplFigs.1-9" is a pdf containing all the Supplementary Figures 1-9 with their legendsThe file "Suppl_Tables_final" is a xlsx with Tables S1-S14 corresponding to all data shown in the Cortot et al. paper.