0000000000335405
AUTHOR
B. Gadea
showing 2 related works from this author
Embryo classification method based in time-lapse analysis is associated with blastocyst quality
2012
groups: 87.7%(10434/11893), 87.4%(2674/3060), 80.2%(1350/1683) and 81.0%(634/783). Abnormal fertilization rate was significantly lower in 9598% group(6.9%,54/783) than other groups(16.1%,1918/11893; 13.8%,421/ 3060; 11.4%,192/1683). Good embryo, pregnancy and implantation rates were no significant differences among four groups(48.9%,61.6%,43.5%; 48.9%,62.1%,43.5%; 50.0%,57.6%,42.3%; 53.1%,62.5%,45.7%). CONCLUSION: Sperm morphology does not affect normal fertilization, embryonic development and clinical outcome.
Comparison of three different staining methods for the assessment of epididymal red deer sperm morphometry by computerized analysis with ISAS®
2005
When collection of ejaculated sperm samples is not possible, as is the case with wild species, the epididymides of sacrificed wild males become the only possible source of spermatozoa. Mature cauda epididymal spermatozoa display characteristics similar to those of ejaculated sperm cells. The present work proposes a sperm staining technique suitable for the morphometric evaluation of red deer epididymal sperm using a new computerized system. Epididymides from wild animals were extracted no later than 2 h post mortem. After epididymal sectioning, sperm samples were collected, cooled to and equilibrated at 5 °C, and frozen in liquid nitrogen. Before staining, sperm samples were thawed for 20 s…