0000000000336602
AUTHOR
Daniela Caporossi
SFRR-E Young Investigator AwardeeαB-crystallin modulation after acute exercise in skeletal muscle: the role of oxidative stress and fiber composition.
αB-crystallin (CRYAB) is a member of the small heat shock proteins implicated in various biological functions, particularly in skeletal muscle where it is involved in adaptive remodelling processes, activation of gene transcription and stabilization of nascent proteins.In this research we analysed αB-crystallin' response in mouse gastrocnemius at 15' and 30' of recovery from an acute aerobic exercise (1hour), correlating its modulation with oxidative stress level and fiber composition, red (RG) and white gastrocnemius (WG).We found for the first time that the acute exercise lead to a short term, specific increase of phospho-αB-crystallin level (pCRYAB) in the RG, while no changes were obser…
The early response of αB-crystallin to a single bout of aerobic exercise in mouse skeletal muscles depends upon fiber oxidative features
Besides its substantial role in eye lens, αB-crystallin (HSPB5) retains fundamental function in striated muscle during physiological or pathological modifications. In this study, we aimed to analyse the cellular and molecular factors driving the functional response of HSPB5 protein in different muscles from mice subjected to an acute bout of non-damaging endurance exercise or in C2C12 myocytes upon exposure to pro-oxidant environment, chosen as “in vivo” and “in vitro” models of a physiological stressing conditions, respectively.To this end, red (GR) and white gastrocnemius (GW), as sources of slow-oxidative and fast-glycolytic/oxidative fibers, as well as the soleus (SOL), mainly composed …
Exercise-Induced Activation and Translocation of αB-Crystallin in Skeletal Muscle Depends upon Fiber Type and Oxidative Stress
Alpha B-crystallin (CRYAB) is a member of the small heat shock proteins implicated in various biological functions, particularly in skeletal muscle tissue [1], where it results to be modulated following exercise-induced reactive oxygen species (ROS) [2]. In this work we aimed to analyse the CRYAB response to acute exercise with respect muscle fiber composition and to identify the underlying molecular mechanism by the utilization of the C2C12 “in vitro” cellular model. Our results highlighted as acute exercise determines a specific increase of phospho-CRYAB both in the red, but not white, gastrocnemius (GS), with an higher amount of oxidative and oxidative-glycolytic fibers, and in soleus (S…
Sex-based differences after a single bout of exercise on PGC1α isoforms in skeletal muscle: A pilot study
To date, there are limited and incomplete data on possible sex-based differences in fiber-types of skeletal muscle and their response to physical exercise. Adult healthy male and female mice completed a single bout of endurance exercise to examine the sex-based differences of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α), heat shock protein 60 (Hsp60), interleukin 6 (IL-6) expression, as well as the Myosin Heavy Chain (MHC) fiber-type distribution in soleus and extensor digitorum longus (EDL) muscles. Our results showed for the first time that in male soleus, a muscle rich of type IIa fibers, endurance exercise activates specifically genes involved in mito…
αB-crystallin activation in cardiac muscle by acute exercise mirrors the sHSP kinetic in oxidative skeletal muscle fibers: animal and cellular study
Alpha-B-Crystallin (CRYAB), a Small Heat Shock Protein sensitive to oxidative stress, is implicated in various biological processes in many tissues. In cardiac muscle, CRYAB exerts a cardio protective role in ischemia-induced damage preventing apoptosis and necrosis. We aimed to study αB-crystallin’ response in mouse cardiac tissue (H), at different time of recovery from an acute aerobic exercise (1 hour), correlating its modulation with oxidative stress level. We found that a single bout exercise lead to a specific short-term increase of phospho-αB-crystallin level (pCRYAB), without changes of its total expression. Further, the level of 4-hydroxynonenal, a marker of lipidic peroxidation, h…
A single bout of endurance exercise induces αB-crystallin (CRYAB) modulation in cardiac muscle as it happens in oxidative skeletal muscle fibers
CRYAB is a small Heat Shock Protein, expressed in various tissues such as skeletal and cardiac muscles, activated as phosphorylated CRYAB (pCRYAB) and involved in several pathophysiological processes. In mammals there are no reports to date on CRYAB activation following an acute endurance exercise, so the aim of my study was to explore in mouse cardiac tissue the pCRYAB levels as effect of this exercise at 0’, 15’ and 120’ of recovery. H2O2 - treated HL-1 cardiomyocytes have been utilized as in vitro model to identify the underlying molecular mechanism/s. Both in vivo and in vitro models showed no changes in CRYAB protein expression level but its phosphorylation state was significantly incr…
Function and Fiber-Type Specific Distribution of Hsp60 and αB-Crystallin in Skeletal Muscles: Role of Physical Exercise
Simple Summary Skeletal muscle represents about 40% of the body mass in humans and it is a copious and plastic tissue, rich in proteins that are subject to continuous rearrangements. Physical exercise is considered a physiological stressor for different organs, in particular for skeletal muscle, and it is a factor able to stimulate the cellular remodeling processes related to the phenomenon of adaptation. All cells respond to various stress conditions by up-regulating the expression and/or activation of a group of proteins called heat shock proteins (HSPs). Although their expression is induced by several stimuli, they are commonly recognized as HSPs due to the first experiments showing thei…
αB-crystallin response to a pro-oxidant non-cytotoxic environment in murine cardiac cells: An "in vitro" and "in vivo" study.
The αB-crystallin (HSPB5) protein is modulated in response to a wide variety of stressors generated by multiple physio-pathological conditions, sustained by reactive oxygen species (ROS) production. In cardiac muscle tissue, this protein regulates various cellular processes, such as protein degradation, apoptosis and the stabilization of cytoskeletal elements. In this work, we studied the role of HSPB5 expression, activation and localization in HL-1 murine cardiomyocytes exposed to pro-oxidant and non-cytotoxic H2O2 concentration, as well as in cardiac tissue isolated from mice following an acute, non-damaging endurance exercise. Our results demonstrated that HSPB5 is the most abundant HSP …