0000000000337189
AUTHOR
J.l. Ruiz-cerdá
Integrative Metabolomic and Transcriptomic Analysis for the Study of Bladder Cancer
Metabolism reprogramming is considered a hallmark of cancer. The study of bladder cancer (BC) metabolism could be the key to developing new strategies for diagnosis and therapy. This work aimed to identify tissue and urinary metabolic signatures as biomarkers of BC and get further insight into BC tumor biology through the study of gene-metabolite networks and the integration of metabolomics and transcriptomics data. BC and control tissue samples (n = 44) from the same patients were analyzed by High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance and microarrays techniques. Besides, urinary profiling study (n = 35) was performed in the same patients to identify a metabolomic profi…
Efecto apoptótico como biomarcador de enfermedad, severidad y seguimiento en la cistitis intersticial
Resumen Objetivo Estudiar si el test de efecto apoptotico podria servir como biomarcador de severidad en el sindrome de dolor vesical/cistitis intersticial. Material y metodos Se realizo un estudio prospectivo entre enero de 2010 y enero de 2015, se incluyeron 57 pacientes diagnosticadas de cistitis intersticial y 49 de dolor pelvico cronico de origen ginecologico. Se expuso la orina a cultivos celulares y se analizo su capacidad para inducir apoptosis en ellos. Posteriormente se llevo acabo un analisis estadistico para valorar si el efecto apoptotico se asociaba con la sintomatologia. Resultados Al realizar un analisis de la asociacion entre el grado del efecto apoptotico y la sintomatolog…
Apoptotic effect as biomarker of disease, severity and follow-up in interstitial cystitis
Objective: To determine whether the apoptotic effect test could serve as a biomarker of severity in bladder pain syndrome/interstitial cystitis. Material and methods: A prospective study was conducted between January 2010 and January 2015, which included 57 patients diagnosed with interstitial cystitis and 49 diagnosed with chronic pelvic pain of gynaecological origin. The urine was exposed to cell cultures, and the urine's capacity for inducing apoptosis in the cultures was analysed. A statistical analysis was then conducted to assess whether the apoptotic effect was associated with the symptoms. Results: After performing an analysis of the association between the degree of apoptotic effec…
Urinary Metabolic Signatures Detect Recurrences in Non-Muscle Invasive Bladder Cancer
Patients with non-muscle invasive bladder cancer (NMIBC) undergo lifelong monitoring based on repeated cystoscopy and urinary cytology due to the high recurrence rate of this tumor. Nevertheless, these techniques have some drawbacks, namely, low accuracy in detection of low-grade tumors, omission of pre-neoplastic lesions and carcinomas in situ (CIS), invasiveness, and high costs. This work aims to identify a urinary metabolomic signature of recurrence by proton Nuclear Magnetic Resonance (1H NMR) spectroscopy for the follow-up of NMIBC patients. To do this, changes in the urinary metabolome before and after transurethral resection (TUR) of tumors are analyzed and a Partial Least Square Dis…
Intratumoral heterogeneity of DNA content in renal cell carcinoma and its prognostic significance
BACKGROUND A multiple sampling study was performed on 124 specimens of renal cell carcinomas to analyze the consistency and reliability of DNA measurements. The authors investigated intratumoral DNA heterogeneity and its role as a adverse prognostic factor for disease progression. METHODS DNA content was analyzed by flow cytometry on three different samples of the same tumor. The Cronbach α coefficient was used to assess the reliability and a Cox proportional hazards model was used to test the effect of DNA ploidy heterogeneity on time of disease progression. RESULTS The agreement among the DNA ploidy samples was high. The number of aneuploid findings increased significantly with the number…
Bladder cancer recurrence surveillance by urine metabolomics analysis.
AbstractNon Muscle Invasive Bladder Cancer (NMIBC) is among the most frequent malignant cancers worldwide. NMIBC is treated by transurethral resection of the bladder tumor (TURBT) and intravesical therapies, and has the highest recurrence rate among solid tumors. It requires a lifelong patient monitoring based on repeated cystoscopy and urinary cytology, both having drawbacks that include lack of sensitivity and specificity, invasiveness and care costs. We conducted an investigative clinical study to examine changes in the urinary metabolome of NMBIC patients before and after TURBT, as well during the subsequent surveillance period. Adjusting by prior probability of recurrence per risk, dis…
Value of Deoxyribonucleic Acid Ploidy and Nuclear Morphometry for Prediction of Disease Progression in Renal Cell Carcinoma
AbstractPurpose: A retrospective study was performed on 108 patients with localized renal cell carcinoma (pT1 to 3a N0M0) to determine whether ploidy and nuclear morphometry are independent predictive factors in addition to stage and grade.Materials and Methods: Deoxyribonucleic acid (DNA) content was analyzed by flow cytometry and nuclear morphometry characterized by 5 nuclear descriptors. A Cox proportional hazards regression model was used to identify significant prognostic factors for disease progression.Results: A model combining tumor stage and grade, DNA ploidy and nuclear minor axis was chosen as optimal with risk of disease progression increased with increasing tumor stage and grad…
Towards the potential use of (1)H NMR spectroscopy in urine samples for prostate cancer detection
A simple method based in multivariate analysis of 1H NMR spectra profiles of urine samples can be used to detect patients with prostate cancer.