0000000000338166
AUTHOR
Vito Porcelli
Toward the Standardization of Mitochondrial Proteomics: The Italian Mitochondrial Human Proteome Project Initiative
The Mitochondrial Human Proteome Project aims at understanding the function of the mitochondrial proteome and its crosstalk with the proteome of other organelles. Being able to choose a suitable and validated enrichment protocol of functional mitochondria, based on the specific needs of the downstream proteomics analysis, would greatly help the researchers in the field. Mitochondrial fractions from ten model cell lines were prepared using three enrichment protocols and analyzed on seven different LC-MS/MS platforms. All data were processed using neXtProt as reference database. The data are available for the Human Proteome Project purposes through the ProteomeXchange Consortium with the iden…
Increasing contextual information by merging existing archaeological data with state of the art laser scanning in the prehistoric funerary deposit of Pastora Cave, Eastern Spain
Abstract In this paper we present a virtual reconstruction of prehistoric funerary practices in Pastora Cave, a collective burial site in Eastern Spain that dates from the Late Neolithic, Chalcolithic and Bronze Age. Modern data of the cave was captured by 3D laser scanning techniques and added to recorded archaeological data and 3D graphic information. The combination of these data sets allowed us to create a hypothetical reconstruction to analyze the material excavated in the 1940s and 50s in greater spatial context. A 3D model of the current cave was created in order to serve as a basis for modeling the relative stratigraphic information available. We present the methodology employed and…