0000000000339029
AUTHOR
Manchikatla Venkat Rajam
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
International audience; Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involv…
Genetic Engineering Strategies for Abiotic Stress Tolerance in Plants
Crop plants are affected by a variety of abiotic stresses such as salinity, drought, extreme temperatures, and oxidative stress and cause a significant yield loss (more than 50 %). In the near future, these abiotic stresses might increase because of global climate change. Abiotic stresses lead to dehydration or osmotic stress through reduced availability of water for vital cellular functions and maintenance of turgor pressure and also result in high production of reactive oxygen species (ROS). Plants are evolved with various mechanisms such as changes in cellular and metabolic processes to cope with the stress condition. Recent developments in molecular genetics have contributed greatly to …