6533b7d9fe1ef96bd126c44a
RESEARCH PRODUCT
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
Archana S. GandheJoanna KotwicaPiotr BebasKostas IatrouJadwiga M. GiebultowiczMagnus LundmarkJianghong LiChoon Wei WeeDaniela NowaraJisheng LiuSriramana KanginakudruMichael R. StrandAntónia MonteiroNirotpal MrinalBernard DuvicMasatoshi IgaL. N. GatehouseAlejandra BravoTeruyuki NiimiShogo MatsumotoAtsushi OhnishiJennie S. GarbuttMario SoberónCristina M. CravaCornelis J. P. GrimmelikhuijzenHanneke HuvenneDwayne D. HegedusDwayne D. HegedusFrank HauserJean Luc AymericChunju AnManchikatla Venkat RajamG FelfoldiGuy SmaggheRobert M. SimpsonHaruhiko FujiwaraAlexie PapanicolaouAlexie PapanicolaouRuud A. De MaagdSalvador HerreroSalvador HerreroLuc SweversIngrid FayeDerek CollingeDerek CollingeFrançois ChevalierSuzanne V. SaenkoShuichiro TomitaRyo FutahashiRyo FutahashiPing WangMaria P. PapakonstantinouDavid G. HeckelMerete AlbrechtsenMartina Meyering-vosKavita BitraAstrid T. GrootSteven HrycajVicencio OostraJavaregowda NagarajuLihua HuangH.s. GatehouseSteven WhyardChangyou LiWenqing ZhangUmut ToprakOlle TereniusKatsuhisa OzakiAndrea BarthelMichael R. KanostMartin A. ErlandsonMartin A. ErlandsonAleksandar PopadicIsabel GómezRichard H. Ffrench-constantJ. Joe HullPeter J. MillichapKarl H.j. GordonIoannis Eleftherianossubject
0106 biological sciencesPhysiology[SDV]Life Sciences [q-bio]Tissue uptakeBioinformatics01 natural sciencesRNA interferenceRNA interferenceDatabases GeneticDelivery methodsCaenorhabditis elegansRegulation of gene expression0303 health sciencesIMMUNE-RESPONSESMANDUCA-SEXTALepidopteraRNA silencingSILKWORM BOMBYX-MORIResearch DesignInsect ProteinsRNA InterferenceMESSENGER-RNAHELICOVERPA-ARMIGERADOUBLE-STRANDED-RNAComputational biologyBiologyLepidoptera genitaliadsRNA properties03 medical and health sciencesBACILLUS-THURINGIENSISSMALL SILENCING RNASGene silencingAnimalsGene SilencingGene030304 developmental biologyRNA Double-StrandedMechanism (biology)fungiBiology and Life SciencesARMYWORM SPODOPTERA-FRUGIPERDAbiology.organism_classificationImmunity Innate010602 entomologyGene Expression RegulationInsect ScienceEpidermisCAENORHABDITIS-ELEGANSGene functiondescription
International audience; Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-02-01 | Journal of insect physiology |