0000000000349583

AUTHOR

Giuseppe Piccione

showing 3 related works from this author

Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells

2018

In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs were isolated by mechanical and enzymatic treatments, and their identity was verified by colony-forming units (CFU) test and by differentiation into cells of mesodermal lineages. The endothelial differentiation was induced by plating another aliquot of cells in EGM-2 medium, enriched with specific endothelial growth factors. Five subcultures were performed. The expression of stemness genes (OCT4, SOX2 and NANOG) was investigated. The presence of CD90 and the absence of the CD45 were evaluated by flow cytometry. The endothelial-like…

0301 basic medicineCellular differentiationSettore VET/09 - Clinica Chirurgica VeterinariaSettore BIO/13 - Biologia Applicataimmunophenotypical analysiCell DifferentiationNanog Homeobox ProteinGeneral MedicineCadherinsFlow CytometryUp-RegulationPlatelet Endothelial Cell Adhesion Molecule-1Endothelial stem cellDrug CombinationsAdipose Tissueembryonic structuresVeterinary (all)ProteoglycansCollagenStem cellHomeobox protein NANOGadipose-derived mesenchymal stem cellDown-RegulationCD146 AntigenBiology03 medical and health sciencesMatrigel assaySOX2Antigens CDAdipose-derived mesenchymal stem cellsAnimalsEndothelial cells differentiationRats WistarImmunophenotypical analysisMatrigelGeneral VeterinaryGene Expression ProfilingSOXB1 Transcription FactorsMesenchymal stem cellEndothelial CellsMesenchymal Stem Cells3T3-L1Molecular biologyAdipose-derived mesenchymal stem cells; Endothelial cells differentiation; Gene expression; Immunophenotypical analysis; Matrigel assay; Rat; Veterinary (all)Culture MediaRats030104 developmental biologyadipose-derived mesenchymal stem cells; endothelial cells differentiation; gene expression; immunophenotypical analysis; matrigel assay; ratLeukocyte Common AntigensThy-1 AntigensRatLamininGene expressionOctamer Transcription Factor-3
researchProduct

Water temperature influences growth and gonad differentiation in European sea bass (Dicentrarchus labrax, L. 1758)

2016

The effect of rearing temperature on gonad differentiation and growth was evaluated in sea bass (Dicentrarchus labrax). One control group (CG, n = 60) and two experimental groups (EG1, n = 30; EG2, n = 30) were selected. CG was reared at 15.0 °C during 10 days post-hatch (dph) and at 19.0 °C throughout the remaining larval and post-larval development. EG1 was exposed to 14.5 °C from 1 to 50 dph, followed by an increase to 20 °C until sampling (176 dph). EG2 was exposed to 14.5 °C from 1 to 37 dph, followed by an increase to 20 °C until sampling (226 dph); 30 fish from CG were randomly sampled at 176 dph (CG1, control for EG1) and 30 fish were sampled at 226 dph (CG2, control for EG2). Weigh…

Male0301 basic medicineendocrine systemmedicine.medical_specialtyGonadgrowthOvaryBiologyFish measurement03 medical and health sciencesFood Animalsgonad differentiationwater temperatureDicentrarchus labrax gonad differentiation growth water temperatureInternal medicineTestismedicineAnimalsDicentrarchus labraxSexual MaturationSea bassSmall AnimalsLarvaurogenital systemEquineOvaryTemperatureWaterbiology.organism_classification030104 developmental biologymedicine.anatomical_structureEndocrinologyWater temperatureLarvaDicentrarchus labrax Gonad differentiation Growth Water temperature Water temperature Bass Female Larva Male Ovary Sexual Maturation Testis Temperature WaterFish <Actinopterygii>BassFemaleAnimal Science and ZoologyDicentrarchus
researchProduct

Gonadal sexual differentiation of European sea bass (Dicentrarchus labrax, L. 1758) of fingerlings in different size classes

2016

This report describes a study of the gonad differentiation in fingerlings of European sea bass (Dicentrarchus labrax) belonging to different size groups. Fishes were divided into four groups according to their weight: Group 1 (4.5 ± 0.7 g), Group 2 (9.2 ± 0.8 g), Group 3 (14.8 ± 1.8 g), and Group 4 (21.8 ± 2.3 g). In all groups, low percentages of undifferentiated or early sexually differentiated fish were found. Higher percentages of fully differentiated ovaries were found in Groups 1 and 3. Fully differentiated testes occurred in 90% of Group 4. Ninety percent of fish in Group 2 had gonads with intratesticular oocytes. This highlights the occurrence of intersexual cases and suggests that …

0301 basic medicineGonadPhysiologysexual differentiationZoologyAquatic ScienceOceanographygonadEuropean sea bafish size03 medical and health sciencesmedicineDicentrarchus labraxSettore BIO/06 - Anatomia Comparata E CitologiaSea bassPhenotypic plasticitySexual differentiationbiology04 agricultural and veterinary sciencesbiology.organism_classificationFishery030104 developmental biologymedicine.anatomical_structureEuropean sea bass dicentrarchus labrax fish size gonad sexual differentiation040102 fisheries0401 agriculture forestry and fisheriesFish <Actinopterygii>Dicentrarchus
researchProduct