0000000000352000

AUTHOR

Imes Petric

Developing and evaluating tools for assessing the impact of low-dose herbicide nicosulfuron on soil microbial diversity and functioning

Today's conventinal agriculture still relies on the general use of pesticides. Even thought this guarantees production capabilities it also causes environmental pollution having impact on non-targeted organisms. Agricultural practices have been recognized by "The European Soil Framework Directive" as a major threat for soil biodiversity. However, without taking into consideration. recent methodological advances in microbiology, pesticide regulation at EU level relying solely on simple C and N mineralization tè-sts•. ln order to cope with this ECOFUN­-MICROBIODIV project .aim to develop and evaluate tools to estimate the impact of herbicide nicosulfuron on the function and population dynamic…

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ECOFUN-MICROBIODIV : an FP7 European project to estimate the ecotoxicological impact of low dose pesticide application in agriculture on soil functional microbial diversity

Soil is hosting a tremendous microbial diversity playing a key-role in a number of soil ecosystemic services including nutrient cycling and filtering. ln the European Soil Framework Directive pesticides are clearly marked as one of the major threats for soil biodiversity and functioning. ln orcier to guarantee minimum effects of pesticide application on soil microbes, pesticicleregistration at EU level (Regulation 2006/388) consiclers the toxicity of pesticides ontci non targefsoil microbes by relying on carbon- and nitrcigen-mineralization tests (OECO 216, 217). However tnese tests do not provicle a comprehensive assessment of pesticides onto soil microbes.-ECOFUN-MICROBIODIV was a project…

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Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

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